Anti-IQ motif containing B1 antibody (ab241197)
Key features and details
- Rabbit polyclonal to IQ motif containing B1
- Suitable for: IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-IQ motif containing B1 antibody
See all IQ motif containing B1 primary antibodies -
Description
Rabbit polyclonal to IQ motif containing B1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP Human
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Immunogen
Synthetic peptide within Human IQ motif containing B1 aa 548-598. The exact sequence is proprietary.
Database link: Q15051 -
Positive control
- IP: HEK-293T whole cell lysate.
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General notes
ab241197 has not performed satisfactorily when used for WB of IQ motif containing B1 in crude preparations (e.g. whole cell lysate). This antibody can be used for WB of enriched (e.g. immunoprecipitated) sources of IQ motif containing B1.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Antibody was affinity purified using an epitope specific to IQ motif containing B1 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunoprecipitation - Anti-IQ motif containing B1 antibody (ab241197)
IQ motif containing B1 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab241197 at 6 µg/reaction. Western blot was performed from the immunoprecipitate using ab241197 at 1 µg/ml.
Lane 1: ab241197 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG IP in HEK-293T whole cell lysate.Detection: Chemiluminescence with exposure time of 30 seconds.
Lysates prepared in NETN buffer.
