Antibodies, Proteins, Kits and Reagents for Life Science

304 886 ₸ Product size

100 µl 304 886 ₸

Order now and get it on Tuesday March 02, 2021

Anti-Caveolin-2 antibody [EPR2220] - Caveolae Marker (ab79397)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR2220] to Caveolin-2 - Caveolae Marker
Suitable for: WB, IHC-P, Flow Cyt, ICC/IF
Reacts with: Human

Product name

Anti-Caveolin-2 antibody [EPR2220] - Caveolae Marker
See all Caveolin-2 primary antibodies
Description

Rabbit monoclonal [EPR2220] to Caveolin-2 - Caveolae Marker
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
IHC-P	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide within Human Caveolin-2. The exact sequence is proprietary.
Database link: P51636
Positive control
- WB: HeLa cell lysate. IHC-P: Human uterus tissue. ICC/IF: HeLa cells. Flow Cyt: HeLa cells.
General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer

pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR2220
Isotype

IgG
Research areas

Western blot - Anti-Caveolin-2 antibody [EPR2220] - Caveolae Marker (ab79397)

Anti-Caveolin-2 antibody [EPR2220] - Caveolae Marker (ab79397) at 1/5000 dilution + HeLa cell lysate at 10 µg

Secondary
Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 18 kDa
Observed band size: 20 kDa
why is the actual band size different from the predicted?
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caveolin-2 antibody [EPR2220] - Caveolae Marker (ab79397)

ab79397 at 1/100 dilution staining Caveolin-2 in human uterus by Immunohistochemistry using paraffin-embedded tissue. Note staining of endothelial cells and smooth muscles.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
Immunocytochemistry/ Immunofluorescence - Anti-Caveolin-2 antibody [EPR2220] - Caveolae Marker (ab79397)

ab79397 at 1/100 dilution staining Caveolin-2 in HeLa cells by Immunofluorescence.
Flow Cytometry - Anti-Caveolin-2 antibody [EPR2220] - Caveolae Marker (ab79397)

Flow cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling Caveolin-2 (red) with ab79397 at a 1/2000 dilution. Cells were fixed with 80% methanol and permeabilized with 0.1% Tween-20. A goat anti-rabbit IgG (Alexa Fluor^® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (ab172730). Blue (unlabeled control) - Cells without incubation with the primary and secondary antibodies.
Anti-Caveolin-2 antibody [EPR2220] - Caveolae Marker (ab79397)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com