Anti-Caveolin-2 antibody [EPR5471] (ab133484)
![Anti-Caveolin-2 antibody [EPR5471] (ab133484)](https://www.abcam.com/ps/products/133/ab133484/Images/ab133484-236065-anti-caveolin-2-antibody-epr5471-immunofluorescence.jpg "Anti-Caveolin-2 antibody [EPR5471] (ab133484)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5471] to Caveolin-2
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Human
Overview
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Product name
Anti-Caveolin-2 antibody [EPR5471]
See all Caveolin-2 primary antibodies -
Description
Rabbit monoclonal [EPR5471] to Caveolin-2 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanWB Human
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Immunogen
Synthetic peptide within Human Caveolin-2 (C terminal). The exact sequence is proprietary.
Database link: P51636 -
Positive control
- HUVEC, HeLa, 3T3-L1, and U87-MG cell lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR5471 -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-Caveolin-2 antibody [EPR5471] (ab133484)
ab133484 stained MCF cells. The cells were 4% formaldehyde fixed for 10 minutes at room temperature and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1hour at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab133484 at 1/100 dilution) overnight at +4°C. The secondary antibody (pseudo-colored green) was Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081) used at a 1/1000 dilution for 1hour at room temperature. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1hour at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.
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![Western blot - Anti-Caveolin-2 antibody [EPR5471] (ab133484)](https://www.abcam.com/ps/products/133/ab133484/Images/ab133484-147134-anti-caveolin-2-antibody-epr5471-western-blot.jpg)
Western blot - Anti-Caveolin-2 antibody [EPR5471] (ab133484)All lanes : Anti-Caveolin-2 antibody [EPR5471] (ab133484) at 1/1000 dilution
Lane 1 : HUVEC cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : 3T3-L1 cell lysate
Lane 4 : U87-MG cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 18 kDa
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![Anti-Caveolin-2 antibody [EPR5471] (ab133484)](https://www.abcam.com/ps/products/133/ab133484/Images/ab133484-3-benefits-of-recombinant-antibodies.png)
Anti-Caveolin-2 antibody [EPR5471] (ab133484)
