Antibodies, Proteins, Kits and Reagents for Life Science

291 484 ₸ Product size

100 µl 291 484 ₸

Order now and get it on Thursday March 04, 2021

Anti-Caveolin-2 antibody [EPR5471] (ab133484)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR5471] to Caveolin-2
Suitable for: WB, ICC/IF
Reacts with: Mouse, Human

Product name

Anti-Caveolin-2 antibody [EPR5471]
See all Caveolin-2 primary antibodies
Description

Rabbit monoclonal [EPR5471] to Caveolin-2
Host species

Rabbit
Tested Applications & Species

Application Species
ICC/IF
Human

WB
Human

See all applications and species data
Immunogen

Synthetic peptide within Human Caveolin-2 (C terminal). The exact sequence is proprietary.
Database link: P51636
Positive control
- HUVEC, HeLa, 3T3-L1, and U87-MG cell lysates.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
ICC/IF	Human
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer

pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR5471
Isotype

IgG
Research areas

Immunocytochemistry/ Immunofluorescence - Anti-Caveolin-2 antibody [EPR5471] (ab133484)

ab133484 stained MCF cells. The cells were 4% formaldehyde fixed for 10 minutes at room temperature and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1hour at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab133484 at 1/100 dilution) overnight at +4°C. The secondary antibody (pseudo-colored green) was Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081) used at a 1/1000 dilution for 1hour at room temperature. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1hour at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.
Western blot - Anti-Caveolin-2 antibody [EPR5471] (ab133484)

All lanes : Anti-Caveolin-2 antibody [EPR5471] (ab133484) at 1/1000 dilution

Lane 1 : HUVEC cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : 3T3-L1 cell lysate
Lane 4 : U87-MG cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 18 kDa
Anti-Caveolin-2 antibody [EPR5471] (ab133484)

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