Human Thrombin Antibody Pair - BSA and Azide free (ab253308)
Key features and details
- Unconjugated capture and detector antibodies
- Adaptable to any antibody pair-based assay format
- Antibody concentration ~ 1 mg/ml
- BSA and azide free buffer - ready for conjugation
- Reacts with: Human
Overview
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Product name
Human Thrombin Antibody Pair - BSA and Azide free
See all Thrombin kits -
Assay type
ELISA set -
Range
140.625 pg/ml - 9000 pg/ml -
Species reactivity
Reacts with: Human -
Product overview
The Antibody Pair can be used to quantify Human Thrombin. BSA and Azide free antibody pairs include unconjugated capture and detector antibodies suitable for sandwich ELISAs. The antibodies are provided at an approximate concentration of 1 mg/ml as measured by the protein A280 method. The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Both capture and detector antibodies are rabbit monoclonal antibodies delivering consistent, specific, and sensitive results.
For additional information on the performance of the antibody pair, see the equivalent SimpleStep ELISA® Kit (ab270210), which uses the same antibodies. However, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits. Please note that the range provided for the pairs is only an estimation based on the performance of the related product using the same antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody pair in your assay.
Download SDS here.
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Tested applications
Suitable for: Sandwich ELISAmore details -
Platform
Reagents
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Carrier free
Yes -
Components 10 x 96 tests Human Thrombin Capture Antibody (unconjugated) 1 x 100µg Human Thrombin Detector Antibody (unconjugated) 1 x 100µg -
Research areas
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Function
Thrombin, which cleaves bonds after Arg and Lys, converts fibrinogen to fibrin and activates factors V, VII, VIII, XIII, and, in complex with thrombomodulin, protein C. Functions in blood homeostasis, inflammation and wound healing. -
Tissue specificity
Expressed by the liver and secreted in plasma. -
Involvement in disease
Factor II deficiency
Ischemic stroke
Thrombophilia due to thrombin defect
Pregnancy loss, recurrent, 2 -
Sequence similarities
Belongs to the peptidase S1 family.
Contains 1 Gla (gamma-carboxy-glutamate) domain.
Contains 2 kringle domains.
Contains 1 peptidase S1 domain. -
Post-translational
modificationsThe gamma-carboxyglutamyl residues, which bind calcium ions, result from the carboxylation of glutamyl residues by a microsomal enzyme, the vitamin K-dependent carboxylase. The modified residues are necessary for the calcium-dependent interaction with a negatively charged phospholipid surface, which is essential for the conversion of prothrombin to thrombin.
N-glycosylated. N-glycan heterogeneity at Asn-121: Hex3HexNAc3 (minor), Hex4HexNAc3 (minor) and Hex5HexNAc4 (major). At Asn-143: Hex4HexNAc3 (minor) and Hex5HexNAc4 (major). -
Cellular localization
Secreted, extracellular space. - Information by UniProt
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Alternative names
- Coagulation factor II
- Coagulation factor II thrombin
- EC 3.4.21.5
see all -
Database links
- Entrez Gene: 2147 Human
- Omim: 176930 Human
- SwissProt: P00734 Human
- Unigene: 655207 Human
Images
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Sandwich ELISA - Human Thrombin Antibody Pair - BSA and Azide free (ab253308)To learn more about the advantages of recombinant antibodies see here.
