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Human GM-CSF ELISA Kit (CSF2) (ab174448)

Key features and details

  • One-wash 90 minute protocol
  • Sensitivity: 3.7 pg/ml
  • Range: 15.625 pg/ml - 1000 pg/ml
  • Sample type: Cell culture media, Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Human

Overview

  • Product name

    Human GM-CSF ELISA Kit (CSF2)
    See all GM-CSF kits

  • Detection method

    Colorimetric

  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Supernatant 8 1.4%
    Inter-assay
    Sample n Mean SD CV%
    Supernatant 3 1.8%

  • Sample type

    Cell culture supernatant, Serum, Cell culture media, Hep Plasma, EDTA Plasma, Cit plasma

  • Assay type

    Sandwich (quantitative)

  • Sensitivity

    3.7 pg/ml

  • Range

    15.625 pg/ml - 1000 pg/ml

  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 100 99% - 102%
    Serum 81 78% - 86%
    Cell culture media 100 98% - 101%
    Hep Plasma 84 84% - 86%
    EDTA Plasma 73 72% - 74%
    Cit plasma 79 77% - 80%

  • Assay time

    1h 30m

  • Assay duration

    One step assay

  • Species reactivity

    Reacts with: Human

  • Product overview

    Human GM-CSF ELISA (ab174448) kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of p53 protein in human serum, plasma and cell culture media. It uses our proprietary SimpleStep ELISA® technology. Quantitate human GM-CSF with 3.7 pg/ml sensitivity.

    SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

            -Single-wash protocol reduces assay time to 90 minutes or less
            -High sensitivity, specificity and reproducibility from superior antibodies
            -Fully validated in biological samples
            -96-wells plate breakable into 12 x 8 wells strips

    A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpeStep ELISA® kits.

  • Notes

    Granulocyte-macrophage colony-stimulating factor (GM-CSF) is a cytokine that stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes. GM-CSF is used therapeutically in myeloid reconstitution following bone marrow transplant, bone marrow transplant engraftment failure or delay, mobilization and following transplantation of autologous peripheral blood progenitor cells, and following induction chemotherapy in older adults with acute myelogenous leukemia (AML).  This kit recognizes Primate and Human GM-CSF which are 95% homologous.

  • Platform

    Microplate

Properties

Images

  • Other - Human GM-CSF ELISA Kit (CSF2) (ab174448)
    Other - Human GM-CSF ELISA Kit (CSF2) (ab174448)

    SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • Example of human GM-CSF standard curve in Sample Diluent NS.
    Example of human GM-CSF standard curve in Sample Diluent NS.

    The GM-CSF standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

  • Interpolated concentrations of spike GM-CSF in human serum, and plasma samples.
    Interpolated concentrations of spike GM-CSF in human serum, and plasma samples.

    The concentrations of GM-CSF were measured in duplicates, interpolated from the GM-CSF standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 50%, plasma (citrate) 50%, plasma (EDTA) 50%, and plasma (heparin) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

  • Interpolated concentrations of native GM-CSF in human cell culture supernatant sample.
    Interpolated concentrations of native GM-CSF in human cell culture supernatant sample.

    The concentrations of GM-CSF were measured in duplicates, interpolated from the GM-CSF standard curves and corrected for sample dilution. Undiluted samples are as follows: Stimulated PBMC Cell Culture Supernatant 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean GM-CSF concentration was determined to be 2599 pg/mL in Stimulated PBMC Cell Culture Supernatant and undetectable in unstimulated PBMC Cell Culture Supernatant.

  • Sandwich ELISA - Human GM-CSF ELISA Kit (CSF2) (ab174448)
    Sandwich ELISA - Human GM-CSF ELISA Kit (CSF2) (ab174448)
    To learn more about the advantages of recombinant antibodies see here.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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