Mouse ICAM2 Matched Antibody Pair Kit (ab215404)
Key features and details
- Unlabeled capture antibody, biotin-labeled detection antibody and calibrated protein standard
- For economical ELISA and ELISA-based assay development
- Reacts with: Mouse
- Range: 62.5 pg/ml - 4000 pg/ml
Overview
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Product name
Mouse ICAM2 Matched Antibody Pair Kit
See all ICAM2 kits -
Detection method
Colorimetric -
Assay type
ELISA set -
Sensitivity
52.7 pg/ml -
Range
62.5 pg/ml - 4000 pg/ml -
Species reactivity
Reacts with: Mouse -
Product overview
Mouse ICAM2 Matched Antibody Pair Kits include a capture and a biotinylated detector antibody pair, along with a calibrated protein standard, suitable for sandwich ELISA. The Matched Antibody Pair Kit can be used to quantify native and recombinant mouse ICAM2.
Optimization of the kit reagents to sample type, immunoassay format or instrumentation may be required. Guidelines for use of this kit in a standard 96-well microplate sandwich ELISA using HRP/TMB system of colorimetric detection is described in this assay procedure for the purposes of quantification.
Protocol information and tips on the use of the Matched Antibody Pair kits for sandwich ELISA can be found on our website. An accessory pack can be purchased which includes buffer reagents required to perform 10 x 96-well plate sandwich ELISAs (ab210905).
For additional information on the performance of the antibody pair used in this kit, please see our equivalent SimpleStep ELISA kit ab205572. Please note that while the antibody pair is the same provided in the corresponding SimpleStep ELISA Kit, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits.
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Tested applications
Suitable for: ELISA, IAmore details -
Platform
Reagents
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 10 x 96 tests 5 x 96 tests Mouse ICAM2 Capture Antibody 2 x 50µg 1 x 50µg Mouse ICAM2 Detector Antibody 2 x 12.5µg 1 x 12.5µg Mouse ICAM2 Lyophilized Protein 2 vials 1 vial -
Research areas
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Relevance
ICAM2 is a member of the intercellular adhesion molecule (ICAM) family. All ICAM proteins are type I transmembrane glycoproteins, contain 2-9 immunoglobulin like C2 type domains, and bind to the leukocyte adhesion LFA1 protein. This protein may play a role in lymphocyte recirculation by blocking LFA1 dependent cell adhesion. It mediates adhesive interactions important for antigen specific immune response, NK cell mediated clearance, lymphocyte recirculation, and other cellular interactions important for immune response and surveillance. -
Cellular localization
Membrane; Single-pass type I membrane protein -
Alternative names
- CD102
- CD102 antigen
- ICAM 2
see all -
Database links
- Entrez Gene: 15896 Mouse
- SwissProt: P35330 Mouse
- Unigene: 394 Mouse
Images
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Mouse ICAM2 standard curve.
Standard calibration curve. Background subtracted values are graphed.
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Sandwich ELISA - Mouse ICAM2 Matched Antibody Pair Kit (ab215404)To learn more about the advantages of recombinant antibodies see here.