Anti-NAK/TBK1 antibody (ab235253)
Key features and details
- Rabbit polyclonal to NAK/TBK1
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-NAK/TBK1 antibody
See all NAK/TBK1 primary antibodies -
Description
Rabbit polyclonal to NAK/TBK1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanWB Mouse
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: NIH/3T3 cell lysate. ICC/IF: HeLa cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-NAK/TBK1 antibody (ab235253)
4% formaldehyde-fixed, 0.3% Triton X-100 permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for NAK/TBK1 (green) using ab235253 at 15 µg/ml overnight at 4⁰C, in ICC/IF, followed by Donkey Anti-Rabbit IgG DyLight™ 488 Conjugated Preadsorbed at 5 ug/ml 1 hour at room temperature. Counter-stained with DAPI.
(A) DAPI
(B) ab235253 + sec Dylight488
(C) Merge A+B
(D) Secondary onlyExpected localization: cytoplasm.
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Western blot - Anti-NAK/TBK1 antibody (ab235253)Anti-NAK/TBK1 antibody (ab235253) at 1/1000 dilution + NIH/3T3 (mouse embyro fibroblast cell line) cell lysate
Secondary
Rabbit IgG HRP at 1/70000 dilution
Predicted band size: 84 kDa
Exposure time: 30 seconds
