Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-NAK/TBK1 (phospho S172) antibody [EPR2867(2)] (ab109272)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR2867(2)] to NAK/TBK1 (phospho S172)
  • Suitable for: WB, Dot blot
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-NAK/TBK1 (phospho S172) antibody [EPR2867(2)]
    See all NAK/TBK1 primary antibodies

  • Description

    Rabbit monoclonal [EPR2867(2)] to NAK/TBK1 (phospho S172)

  • Host species

    Rabbit

  • Specificity

    This antibody only detects NAK/TBK1 phosphorylated at serine 172.

  • Tested Applications & Species

    Application Species
    WB
    Mouse
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
    (Peptide available as ab206124)

  • Positive control

    • HeLa cell lysate, treated with Calyculin A.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Dot Blot - Anti-NAK/TBK1 (phospho S172) antibody [EPR2867(2)] (ab109272)
    Dot Blot - Anti-NAK/TBK1 (phospho S172) antibody [EPR2867(2)] (ab109272)

    Dot blot analysis of NAK/TBK1 (phospho S172) phospho peptide (Lane 1) and NAK/TBK1 non-phospho peptide (Lane 2) labelling NAK/TBK1 (phospho S172) phospho peptide with ab109272 at a dilution of 1:1000 dilution (0.627μg/ml). A Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) was used as the secondary antibody at a dilution of 1:20,000 dilution.

    Blocking buffer: 5% NFDM/TBST.
    Dilution buffer: 5% NFDM /TBST .

  • Western blot - Anti-NAK/TBK1 (phospho S172) antibody [EPR2867(2)] (ab109272)
    Western blot - Anti-NAK/TBK1 (phospho S172) antibody [EPR2867(2)] (ab109272)
    All lanes : Anti-NAK/TBK1 (phospho S172) antibody [EPR2867(2)] (ab109272) at 1/5000 dilution

    Lane 1 : Untreated NIH/3T3 (mouse embryo) whole cell lysate
    Lane 2 : NIH/3T3 (mouse embryo) treated with 10mM pervanadate for 30 minutes whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution

    Predicted band size: 84 kDa



    Blocking buffer and diluting buffer: 5% NFDM /TBST

  • Western blot - Anti-NAK/TBK1 (phospho S172) antibody [EPR2867(2)] (ab109272)
    Western blot - Anti-NAK/TBK1 (phospho S172) antibody [EPR2867(2)] (ab109272)
    All lanes : Anti-NAK/TBK1 (phospho S172) antibody [EPR2867(2)] (ab109272) at 1/5000 dilution

    Lane 1 : HeLa (human cervix adenocarcinoma) treated with Calyculin A whole cell lysate
    Lane 2 : Untreated HeLa (human cervix adenocarcinoma) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution

    Predicted band size: 84 kDa
    Additional bands at: 84 kDa. We are unsure as to the identity of these extra bands.



    Blocking buffer and diluting buffer: 5% NFDM/TBST

  • Western blot - Anti-NAK/TBK1 (phospho S172) antibody [EPR2867(2)] (ab109272)
    Western blot - Anti-NAK/TBK1 (phospho S172) antibody [EPR2867(2)] (ab109272)
    All lanes : Anti-NAK/TBK1 (phospho S172) antibody [EPR2867(2)] (ab109272) at 1/1000 dilution

    Lane 1 : HeLa cell lysate, untreated
    Lane 2 : HeLa cell lysate, treated with Calyculin A

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 84 kDa

  • Anti-NAK/TBK1 (phospho S172) antibody [EPR2867(2)] (ab109272)
    Anti-NAK/TBK1 (phospho S172) antibody [EPR2867(2)] (ab109272)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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