Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-SIK2 antibody [EPR22722-23] - BSA and Azide free (ab280575)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22722-23] to SIK2 - BSA and Azide free
  • Suitable for: WB
  • Reacts with: Mouse

Overview

  • Product name

    Anti-SIK2 antibody [EPR22722-23] - BSA and Azide free
    See all SIK2 primary antibodies

  • Description

    Rabbit monoclonal [EPR22722-23] to SIK2 - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Mouse
    Recombinant fragment
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Untreated 3T3-L1, 3T3-L1 differentiation for 6 days, Mouse P70 brown adipose, P70 white adipose, brain,293T transfected with a DDDDK-tagged mouse SIK2(WT) expression vector lysates.

  • General notes

    ab280575 is the carrier-free version of ab245211. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab280575 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-SIK2 antibody [EPR22722-23] - BSA and Azide free (ab280575)
    Western blot - Anti-SIK2 antibody [EPR22722-23] - BSA and Azide free (ab280575)
    All lanes : Anti-SIK2 antibody [EPR22722-23] (ab245211) at 1/1000 dilution

    Lane 1 : Untreated 3T3-L1 (mouse embryonic fibroblast) whole cell lysate
    Lane 2 : 3T3-L1 differentiation for 6 days whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size: 104 kDa



    This data was developed using ab245211, the same antibody clone in a different buffer formulation. 

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    The expression profile observed is consistent with what has been described in the literature (PMID: 12624099)

     Exposure time: 3 minutes

     

  • Western blot - Anti-SIK2 antibody [EPR22722-23] - BSA and Azide free (ab280575)
    Western blot - Anti-SIK2 antibody [EPR22722-23] - BSA and Azide free (ab280575)
    All lanes : Anti-SIK2 antibody [EPR22722-23] (ab245211) at 1/1000 dilution

    Lane 1 : Mouse P70 brown adipose tissue lysate
    Lane 2 : Mouse P70 white adipose tissue lysate
    Lane 3 : Mouse brain tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size: 104 kDa



    This data was developed using ab245211, the same antibody clone in a different buffer formulation. 

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

     Exposure time: Lanes 1-2: 59 seconds; Lane 3: 3 minutes.

     

  • Western blot - Anti-SIK2 antibody [EPR22722-23] - BSA and Azide free (ab280575)
    Western blot - Anti-SIK2 antibody [EPR22722-23] - BSA and Azide free (ab280575)
    All lanes : Anti-SIK2 antibody [EPR22722-23] (ab245211) at 1/1000 dilution

    Lane 1 : 293T transfected with a DDDDK-tagged mouse SIK1(WT) expression vector, whole cell lysate
    Lane 2 : 293T (human embryonic kidney) transfected with an empty vector (vector control) containing a DDDDK tag, whole cell lysate
    Lane 3 : 293T transfected with a DDDDK-tagged mouse SIK2(WT) expression vector, whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size: 104 kDa



    This data was developed using ab245211, the same antibody clone in a different buffer formulation. 

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    This antibody doesn't react with SIK1.

     Exposure time: 3 seconds

     

  • Anti-SIK2 antibody [EPR22722-23] - BSA and Azide free (ab280575)
    Anti-SIK2 antibody [EPR22722-23] - BSA and Azide free (ab280575)

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