Anti-IKK alpha + IKK beta (phospho S176 + S177) antibody (ab194528)
Key features and details
- Rabbit polyclonal to IKK alpha + IKK beta (phospho S176 + S177)
- Suitable for: WB
- Reacts with: Mouse
- Isotype: IgG
Overview
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Product name
Anti-IKK alpha + IKK beta (phospho S176 + S177) antibody
See all IKK alpha + IKK beta primary antibodies -
Description
Rabbit polyclonal to IKK alpha + IKK beta (phospho S176 + S177) -
Host species
Rabbit -
Specificity
ab194528 detects endogenous level of IKK alpha + IKK beta only when phosphorylated at serine 176/177. -
Tested Applications & Species
See all applications and species dataApplication Species WB Mouse
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Immunogen
Synthetic peptide corresponding to Human IKK alpha + IKK beta (phospho S176 + S177) conjugated to Keyhole Limpet Haemocyanin (KLH). Peptide sequence around phosphorylation site of serine 176/177 (Q-G-S(p)-L-C) derived from Human IKK alpha + IKK beta. SwissProt: O15111/O14920.
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Positive control
- WB: NIH 3T3 cell extracts, treated with TNF.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 49% PBS
PBS (without Mg2+and Ca2+) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab194528 was purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography using non-phosphopeptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-IKK alpha + IKK beta (phospho S176 + S177) antibody (ab194528)All lanes : Anti-IKK alpha + IKK beta (phospho S176 + S177) antibody (ab194528) at 1/500 dilution
Lane 1 : NIH 3T3 cell extract, treated with TNF
Lane 2 : NIH 3T3 cell extract, treated with TNF with antigen-specific peptide
Predicted band size: 85 kDa
