Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-ALKBH1 antibody [EPR6176] (ab126596)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR6176] to ALKBH1
  • Suitable for: WB, IP, IHC-P
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-ALKBH1 antibody [EPR6176]
    See all ALKBH1 primary antibodies

  • Description

    Rabbit monoclonal [EPR6176] to ALKBH1

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human ALKBH1 aa 350 to the C-terminus. The exact sequence is proprietary.

  • Positive control

    • WB: Jurkat, K562 and A549 cell lysates. IHC-P: Human kidney and liver tissues. IP: Jurkat and K562 cell lysates.

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.

  • Dissociation constant (KD)

    KD = 2.80 x 10 -11 M
    Learn more about KD

  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR6176

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-ALKBH1 antibody [EPR6176] (ab126596)
    Western blot - Anti-ALKBH1 antibody [EPR6176] (ab126596)
    All lanes : Anti-ALKBH1 antibody [EPR6176] (ab126596) at 1/1000 dilution

    Lane 1 : Wild-type HAP1 whole cell lysate
    Lane 2 : ALKBH1 knockout HAP1 whole cell lysate
    Lane 3 : K562 whole cell lysate
    Lane 4 : A549 whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Predicted band size: 43 kDa
    Observed band size: 43 kDa



    Lanes 1 - 4: Merged signal (red and green). Green - ab126596 observed at 43 kDa. Red - loading control, ab8245, observed at 37 kDa.

    ab126596 was shown to specifically react with  in wild-type HAP1 cells as signal was lost in ALKBH1 knockout cells. Wild-type and ALKBH1 knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% Milk. Ab126596 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-ALKBH1 antibody [EPR6176] (ab126596)
    Western blot - Anti-ALKBH1 antibody [EPR6176] (ab126596)
    All lanes : Anti-ALKBH1 antibody [EPR6176] (ab126596) at 1/10000 dilution (purified)

    Lane 1 : Jurkat whole cell lysate
    Lane 2 : K562 whole cell lysate
    Lane 3 : A549 whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 43 kDa
    Observed band size: 43 kDa



    Blocking and dilution buffer: 5% NFDM/TBST
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALKBH1 antibody [EPR6176] (ab126596)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALKBH1 antibody [EPR6176] (ab126596)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver tissue labelling ALKBH1 with purified ab126596 at a dilution of 1/50. Heat mediated antigen retrieval was performed using EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

  • Immunoprecipitation - Anti-ALKBH1 antibody [EPR6176] (ab126596)
    Immunoprecipitation - Anti-ALKBH1 antibody [EPR6176] (ab126596)

    ab126596 (purified) at a dilution of 1/30 immunoprecipitating ALKBH1 in Jurkat whole cell lysate.

    Lane 1 (input): Jurkat whole cell lysate (10µg)

    Lane 2 (+): ab126596 + Jurkat whole cell lysate.

    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab126596 in Jurkat whole cell lysate.

    For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

  • Immunoprecipitation - Anti-ALKBH1 antibody [EPR6176] (ab126596)
    Immunoprecipitation - Anti-ALKBH1 antibody [EPR6176] (ab126596)

    ab126596 (purified) at a dilution of 1/30 immunoprecipitating ALKBH1 in K562 whole cell lysate.

    Lane 1 (input): K562 whole cell lysate (10µg)

    Lane 2 (+): ab126596 + K562 whole cell lysate.

    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab126596 in K562 whole cell lysate.

    For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

  • Western blot - Anti-ALKBH1 antibody [EPR6176] (ab126596)
    Western blot - Anti-ALKBH1 antibody [EPR6176] (ab126596)
    All lanes : Anti-ALKBH1 antibody [EPR6176] (ab126596) at 1/1000 dilution (unpurified)

    Lane 1 : Jurkat cell lysate
    Lane 2 : K562 cell lysate
    Lane 3 : A549 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution

    Predicted band size: 43 kDa
    Observed band size: 43 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALKBH1 antibody [EPR6176] (ab126596)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALKBH1 antibody [EPR6176] (ab126596)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling ALKBH1 with unpurified ab126596 at a dilution of 1/50.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • OI-RD Scanning - Anti-ALKBH1 antibody [EPR6176] (ab126596)
    OI-RD Scanning - Anti-ALKBH1 antibody [EPR6176] (ab126596)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-ALKBH1 antibody [EPR6176] (ab126596)
    Anti-ALKBH1 antibody [EPR6176] (ab126596)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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