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Astana Biomed Group, an authorized Abcam distributor in Central Asia

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Anti-Chk1 antibody (ab137400)

Anti-Chk1 antibody (ab137400)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to Chk1
  • Suitable for: WB, ICC/IF, IHC-P
  • Reacts with: Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-Chk1 antibody
    See all Chk1 primary antibodies
  • Description

    Rabbit polyclonal to Chk1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IF, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide, corresponding to a region within the internal sequence amino acids 259-351 of Human Chk1 (Uniprot ID O14757).

  • Positive control

    • 293T nuclear extract, A431, H1299, Molt-4, Raji, HeLa cells, SCM-1 xenograft tissue
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 79.99% PBS, 20% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • DNA Damage & Repair
    • DNA Damage Response
    • Chk1 / Chk2
    • Cancer
    • Cell cycle
    • Kinases/phosphatases
    • Other

Images

  • Western blot - Anti-Chk1 antibody (ab137400)
    Western blot - Anti-Chk1 antibody (ab137400)
    Anti-Chk1 antibody (ab137400) at 1/1000 dilution + 293T nuclear extract at 20 µg

    Predicted band size: 54 kDa



    7.5% SDS PAGE
  • Immunocytochemistry/ Immunofluorescence - Anti-Chk1 antibody (ab137400)
    Immunocytochemistry/ Immunofluorescence - Anti-Chk1 antibody (ab137400)
    Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells labelled with ab137400 at 1/200 dilution. Costained with Hoechst 33342.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Chk1 antibody (ab137400)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Chk1 antibody (ab137400)
    Immunohistochemical analysis of paraffin embedded SCM-1 xenograft tissue labelled with ab137400 at 1/100 dilution.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-Chk1 antibody (ab137400)

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    Alexa Fluor® 488 Anti-Chk1 antibody [EP691Y] (ab196308)

    Applications: ICC/IF

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    Anti-Chk1 (phospho S296) antibody [EPR915] - BSA and Azide free (ab176313)

    Applications: WB

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    Alexa Fluor® 647 Anti-Chk1 antibody [EP691Y] (ab196520)

    Applications: ICC/IF

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    Anti-Chk1 antibody [E250] (ab32531)

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    Anti-Chk1 antibody [EPR3952] (ab133277)

    Applications: WB

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    Anti-Chk1 (phospho S296) antibody [EPR915] (ab79758)

    Applications: WB

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