Anti-Chk1 antibody [E250] (ab32531)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [E250] to Chk1
- Suitable for: IP, WB, Flow Cyt
- Reacts with: Mouse, Rat, Human
Overview
-
Product name
Anti-Chk1 antibody [E250]
See all Chk1 primary antibodies -
Description
Rabbit monoclonal [E250] to Chk1 -
Host species
Rabbit -
Specificity
ab32531 recognises Chk1. -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanICC HumanIP HumanWB MouseHuman -
Immunogen
Synthetic peptide within Human Chk1 aa 1-100 (N terminal). The exact sequence is proprietary.
-
Positive control
- IP: K-562 whole cell lysate. ICC: HeLa cells. Flow Cyt: K-562 cells. WB: K-562, Hela, NIH/3T3, MEF and PC-12 whole cell lysates
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
-
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
E250 -
Isotype
IgG -
Research areas
Images
-
All lanes : Anti-Chk1 antibody [E250] (ab32531) at 1/10000 dilution (Purified)
Lane 1 : MEF (Mouse embryonic fibroblast (immortalized)) whole cell lysate
Lane 2 : PC-12 (Rat adrenal gland pheochromocytoma ) whole cell lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)
Predicted band size: 54 kDa
Observed band size: 54 kDaWe are unsure how to define the extra bands.
-
All lanes : Purfiied at 1/1000 dilution
Lane 1 : K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysate at 1/20 dilution
Lane 2 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg
Lane 3 : NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate at 20 µg
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)
Predicted band size: 54 kDa
Observed band size: 54 kDaWe are unsure how to define the extra bands.
-
Purified ab32531 at 1/20 dilution (1µg) immunoprecipitating Chk1 in K-562 whole cell lysate.
Lane 1 (input): K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysate 10µg
Lane 2 (+): ab32531 + K-562 whole cell lysate.
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab32531 in K-562 whole cell lysate.
VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
Blocking Buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM/TBST.
Observed band size: 54 kDa -
Immunocytochemistry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling Chk1 with purified ab32531 at 1/50 dilution (5.06 µg/mL). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/mL) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
-
Flow Cytometry analysis of K-562 (Human chronic myelogenous leukemia lymphoblast) cells labeling Chk1 with purified ab32531 at 1/30 dilution (10 µg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
-