Anti-Chk1 (phospho S317) antibody (ab38518)
Key features and details
- Rabbit polyclonal to Chk1 (phospho S317)
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Chk1 (phospho S317) antibody
See all Chk1 primary antibodies -
Description
Rabbit polyclonal to Chk1 (phospho S317) -
Host species
Rabbit -
Specificity
Chk1(Phospho-Ser317) Antibody detects endogenous levels of Chk1 only when phosphorylated at Serine 317. -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic phosphopeptide derived from human Chk1 around the phosphorylation site of Serine 317.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, PBS
Without Mg2+ and Ca2+ -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab38518 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Chk1 (phospho S317) antibody (ab38518)All lanes : Anti-Chk1 (phospho S317) antibody (ab38518) at 1/500 dilution
Lane 1 : Extracts from MCF7 cells (5-30ug of total protein)
Lane 2 : Extracts from COLO cells (5-30ug of total protein)
Secondary
All lanes : Alkaline Phosphatase Goat Anti Rabbit IgG (H+L)
Predicted band size: 54 kDa
Observed band size: 63 kDa why is the actual band size different from the predicted?
