Anti-Chk1 (phospho S296) antibody [EPR915] (ab79758)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR915] to Chk1 (phospho S296)
- Suitable for: WB
- Reacts with: Human
Overview
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Product name
Anti-Chk1 (phospho S296) antibody [EPR915]
See all Chk1 primary antibodies -
Description
Rabbit monoclonal [EPR915] to Chk1 (phospho S296) -
Host species
Rabbit -
Specificity
The antibody only detects Chk1 phosphorylated at Serine 296. -
Tested Applications & Species
See all applications and species dataApplication Species WB Human -
Immunogen
Synthetic peptide within Human Chk1. The exact sequence is proprietary.
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Positive control
- 283T cell lysate treated with UV and AP; HeLa cells.
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.1% BSA, 9.85% Tris glycine -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR915 -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-Chk1 (phospho S296) antibody [EPR915] (ab79758) at 1/1500 dilution
Lane 1 : Untreated 293 cell lysate
Lane 2 : Treated with Calyculin A 293 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/1000 dilution
Predicted band size: 54 kDa
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All lanes : Anti-Chk1 (phospho S296) antibody [EPR915] (ab79758) at 1/1000 dilution (Unpurified)
Lane 1 : Untreated 293 cell Lysate
Lane 2 : Treated with Calyculin 293 cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/1000 dilution
Predicted band size: 54 kDa
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