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Anti-Chk1 (phospho S296) antibody [EPR915] (ab79758)

Price and availability

318 288 ₸

Availability

Order now and get it on Thursday March 04, 2021

Anti-Chk1 (phospho S296) antibody [EPR915] (ab79758)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR915] to Chk1 (phospho S296)
  • Suitable for: WB
  • Reacts with: Human

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Overview

  • Product name

    Anti-Chk1 (phospho S296) antibody [EPR915]
    See all Chk1 primary antibodies
  • Description

    Rabbit monoclonal [EPR915] to Chk1 (phospho S296)
  • Host species

    Rabbit
  • Specificity

    The antibody only detects Chk1 phosphorylated at Serine 296.
  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide within Human Chk1. The exact sequence is proprietary.

  • Positive control

    • 283T cell lysate treated with UV and AP; HeLa cells.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.20
    Preservative: 0.05% Sodium azide
    Constituents: 40% Glycerol (glycerin, glycerine), 0.1% BSA, 9.85% Tris glycine
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR915
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • DNA Damage & Repair
    • DNA Damage Response
    • Chk1 / Chk2
    • Cancer
    • Cell cycle
    • Kinases/phosphatases
    • Other

Images

  • Western blot - Anti-Chk1 (phospho S296) antibody [EPR915] (ab79758)
    Western blot - Anti-Chk1 (phospho S296) antibody [EPR915] (ab79758)
    All lanes : Anti-Chk1 (phospho S296) antibody [EPR915] (ab79758) at 1/1500 dilution

    Lane 1 : Untreated 293 cell lysate
    Lane 2 : Treated with Calyculin A 293 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/1000 dilution

    Predicted band size: 54 kDa

  • Western blot - Anti-Chk1 (phospho S296) antibody [EPR915] (ab79758)
    Western blot - Anti-Chk1 (phospho S296) antibody [EPR915] (ab79758)
    All lanes : Anti-Chk1 (phospho S296) antibody [EPR915] (ab79758) at 1/1000 dilution (Unpurified)

    Lane 1 : Untreated 293 cell Lysate
    Lane 2 : Treated with Calyculin 293 cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/1000 dilution

    Predicted band size: 54 kDa

  • Anti-Chk1 (phospho S296) antibody [EPR915] (ab79758)
    Anti-Chk1 (phospho S296) antibody [EPR915] (ab79758)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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