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Anti-Amyloid Precursor Protein antibody [14D6] - BSA and Azide free (ab255770)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rat monoclonal [14D6] to Amyloid Precursor Protein - BSA and Azide free
  • Suitable for: WB, ELISA
  • Reacts with: Rat, Human, Recombinant fragment

Overview

  • Product name

    Anti-Amyloid Precursor Protein antibody [14D6] - BSA and Azide free
    See all Amyloid Precursor Protein primary antibodies

  • Description

    Rat monoclonal [14D6] to Amyloid Precursor Protein - BSA and Azide free

  • Host species

    Rat

  • Tested Applications & Species

    Application Species
    ELISA
    Recombinant fragment
    WB
    Rat
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human and rat hippocampus tissue lysate.

  • General notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    ab255770 is the carrier-free version of ab252814. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab255770 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

Images

  • Western blot - Anti-Amyloid Precursor Protein antibody [14D6] - BSA and Azide free (ab255770)
    Western blot - Anti-Amyloid Precursor Protein antibody [14D6] - BSA and Azide free (ab255770)
    All lanes : Anti-Amyloid Precursor Protein antibody [14D6] (ab252814) at 2.308 µg/ml

    Lane 1 : Human hippocampus tissue lysate
    Lane 2 : Rat hippocampus tissue lysate

    Lysates/proteins at 40 mg/ml per lane.

    Secondary
    All lanes : Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/10000 dilution

    Predicted band size: 86 kDa



    Samples are non-boiled as boiling may cause protein aggregates.

    Exposure times: Lane 1: 15 secs; Lane 2: 92 secs.

    Blocking/Dilution buffer: 5% NFDM/TBST.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252814).

  • ELISA - Anti-Amyloid Precursor Protein antibody [14D6] - BSA and Azide free (ab255770)
    ELISA - Anti-Amyloid Precursor Protein antibody [14D6] - BSA and Azide free (ab255770)

    ELISA - Anti-Amyloid Precursor Protein antibody [14D6] (ab252814).

    ab252814 used at 0-4000 ng/ml.

    Antigen (Human Amyloid Precursor Protein) used at 1000 ng/ml.

    Secondary Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rat IgG (H+L) used at a 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252814).

  • Anti-Amyloid Precursor Protein antibody [14D6] - BSA and Azide free (ab255770)
    Anti-Amyloid Precursor Protein antibody [14D6] - BSA and Azide free (ab255770)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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