Anti-Amyloid Precursor Protein antibody [14D6] - BSA and Azide free (ab255770)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rat monoclonal [14D6] to Amyloid Precursor Protein - BSA and Azide free
- Suitable for: WB, ELISA
- Reacts with: Rat, Human, Recombinant fragment
Overview
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Product name
Anti-Amyloid Precursor Protein antibody [14D6] - BSA and Azide free
See all Amyloid Precursor Protein primary antibodies -
Description
Rat monoclonal [14D6] to Amyloid Precursor Protein - BSA and Azide free -
Host species
Rat -
Tested Applications & Species
See all applications and species dataApplication Species ELISA Recombinant fragmentWB RatHuman -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Human and rat hippocampus tissue lysate.
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
ab255770 is the carrier-free version of ab252814. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab255770 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Ion Exchange Chromatography -
Clonality
Monoclonal -
Clone number
14D6 -
Isotype
IgG2a -
Research areas
Images
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All lanes : Anti-Amyloid Precursor Protein antibody [14D6] (ab252814) at 2.308 µg/ml
Lane 1 : Human hippocampus tissue lysate
Lane 2 : Rat hippocampus tissue lysate
Lysates/proteins at 40 mg/ml per lane.
Secondary
All lanes : Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/10000 dilution
Predicted band size: 86 kDaSamples are non-boiled as boiling may cause protein aggregates.
Exposure times: Lane 1: 15 secs; Lane 2: 92 secs.
Blocking/Dilution buffer: 5% NFDM/TBST.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252814).
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ELISA - Anti-Amyloid Precursor Protein antibody [14D6] (ab252814).
ab252814 used at 0-4000 ng/ml.
Antigen (Human Amyloid Precursor Protein) used at 1000 ng/ml.
Secondary Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rat IgG (H+L) used at a 1/1000 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252814).
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