Antibodies, Proteins, Kits and Reagents for Life Science

Anti-HLA-DPB1 antibody [SP229] - BSA and Azide free (ab246351)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [SP229] to HLA-DPB1 - BSA and Azide free
Suitable for: ICC/IF, WB, Flow Cyt (Intra), IHC-P
Reacts with: Human

Product name

Anti-HLA-DPB1 antibody [SP229] - BSA and Azide free
See all HLA-DPB1 primary antibodies
Description

Rabbit monoclonal [SP229] to HLA-DPB1 - BSA and Azide free
Host species

Rabbit
Tested applications

Suitable for: ICC/IF, WB, Flow Cyt (Intra), IHC-Pmore details
Species reactivity

Reacts with: Human
Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- IHC-P: Human tonsil tissue; Flow Cyt (intra): Ramos cells; ICC: Ramos cells.
General notes
ab246351 is the carrier-free version of ab227676.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C. Do Not Freeze.
Storage buffer

pH: 7.20
Constituent: PBS
Carrier free

Yes
Concentration information loading...
Purity

Protein A/G purified
Purification notes

Purified from TCS by Protein A/G.
Clonality

Monoclonal
Clone number

SP229
Isotype

IgG
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA-DPB1 antibody [SP229] - BSA and Azide free (ab246351)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human tonsil tissue sections labeling HLA-DPB1 with ab227676 at 1/100 dilution (1.10 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227676)
Immunocytochemistry/ Immunofluorescence - Anti-HLA-DPB1 antibody [SP229] - BSA and Azide free (ab246351)

Immunocytochemistry/ Immunofluorescence analysis of Ramos (human Burkitt's lymphoma B lymphocyte) cells labeling HLA-DPB1 with purified ab227676 at 1/10 (10 µg/ml). Cells were fixed in 100% Methanol and permeabilized with None. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) 1/200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227676).
Flow Cytometry - Anti-HLA-DPB1 antibody [SP229] - BSA and Azide free (ab246351)

Flow Cytometry analysis of Ramos (Human Burkitt's lymphoma B lymphocyte) cells labeling HLA-DPB1 with purified ab227676 at 1/20 dilution (5.5 µg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor^® 488, ab150081) secondary antibody was used at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (ab172730) / Black. Unlabeled control - Unlabelled cells / blue.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227676).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA-DPB1 antibody [SP229] - BSA and Azide free (ab246351)

Formalin-fixed, paraffin-embedded human tonsil tissue stained for HLA-DPB1 using ab227676 at 1/100 dilution in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab227676).
Flow Cytometry - Anti-HLA-DPB1 antibody [SP229] - BSA and Azide free (ab246351)

Flow cytometric analysis of Ramos (human Burkitt's lymphoma cell line) cell line labeling HLA-DPB1 with ab227676 at 1/400 dilution (green) compared with a negative control of rabbit IgG (blue).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab227676).
Anti-HLA-DPB1 antibody [SP229] - BSA and Azide free (ab246351)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com