Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-HLA-DPB1 antibody [EPR23947-1] - BSA and Azide free (ab278084)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23947-1] to HLA-DPB1 - BSA and Azide free
  • Suitable for: IHC-P, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-HLA-DPB1 antibody [EPR23947-1] - BSA and Azide free
    See all HLA-DPB1 primary antibodies

  • Description

    Rabbit monoclonal [EPR23947-1] to HLA-DPB1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, WBmore details
    Unsuitable for: Flow Cyt,ICC or IP

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Daudi whole cell lysate; Human tonsil and spleen tissue lysates; HEK-293T transfected with HLA-DPB1 expression vector containing a myc-His-tag®, whole cell lysate. IHC-P: Human tonsil, liver and epityphlon tissue.

  • General notes

    ab278084 is the carrier-free version of ab259802. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab278084 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA-DPB1 antibody [EPR23947-1] - BSA and Azide free (ab278084)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA-DPB1 antibody [EPR23947-1] - BSA and Azide free (ab278084)

    This data was developed using ab259802, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling HLA-DPB1 with ab259802 at 1/2000 (0.286 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Positive staining on human tonsil. The section was incubated with ab259802 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond® Polymer Refine Detection).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Western blot - Anti-HLA-DPB1 antibody [EPR23947-1] - BSA and Azide free (ab278084)
    Western blot - Anti-HLA-DPB1 antibody [EPR23947-1] - BSA and Azide free (ab278084)
    All lanes : Anti-HLA-DPB1 antibody [EPR23947-1] (ab259802) at 1/1000 dilution

    Lane 1 : Daudi (Human Burkitt's lymphoma lymphoblast), whole cell lysate
    Lane 2 : HeLa (human cervix adenocarcinoma epithelial cell ), whole cell lysate
    Lane 3 : Human tonsil tissue lysate
    Lane 4 : Human spleen tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution

    Predicted band size: 29 kDa
    Observed band size: 29 kDa



    This data was developed using ab259802, the same antibody clone in a different buffer formulation. 

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Negative control: HeLa.

    The expression profile/molecular weight observed is consistent with what has been described in the literature. Band detected around 54 kDa could be the dimeric form (PMID: 20959457).

    This blot was developed using a higher sensitivity ECL substrate.

    Exposure times: Lane 1: 81 seconds.

    Lane 2-4: 3 minutes.

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA-DPB1 antibody [EPR23947-1] - BSA and Azide free (ab278084)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA-DPB1 antibody [EPR23947-1] - BSA and Azide free (ab278084)

    This data was developed using ab259802, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded human liver tissue labeling HLA-DPB1 with ab259802 at 1/2000 (0.286 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Positive staining on Kupffer cells in human liver. The section was incubated with ab259802 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond® Polymer Refine Detection).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Western blot - Anti-HLA-DPB1 antibody [EPR23947-1] - BSA and Azide free (ab278084)
    Western blot - Anti-HLA-DPB1 antibody [EPR23947-1] - BSA and Azide free (ab278084)
    All lanes : Anti-HLA-DPB1 antibody [EPR23947-1] (ab259802) at 1/1000 dilution

    Lane 1 : His-tagged human HLA class II histocompatibility antigen, DRB1 beta chain recombinant protein (aa 36-222)
    Lane 2 : HEK-293T transfected with HLA-DPB1 expression vector containing a myc-His-tag®, whole cell lysate 10

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution

    Predicted band size: 29 kDa



    This data was developed using ab259802, the same antibody clone in a different buffer formulation. 

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

     

    Exposure time: 3 minutes.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA-DPB1 antibody [EPR23947-1] - BSA and Azide free (ab278084)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA-DPB1 antibody [EPR23947-1] - BSA and Azide free (ab278084)

    This data was developed using ab259802, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded human epityphlon tissue labeling HLA-DPB1 with ab259802 at 1/2000 (0.286 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Positive staining on human epityphlon. The section was incubated with ab259802 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond® Polymer Refine Detection).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Anti-HLA-DPB1 antibody [EPR23947-1] - BSA and Azide free (ab278084)
    Anti-HLA-DPB1 antibody [EPR23947-1] - BSA and Azide free (ab278084)

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