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Cancer Cell cycle Cell cycle inhibitors Rb

Anti-S6K1 antibody [EPR13429] - BSA and Azide free (ab250904)

Anti-S6K1 antibody [EPR13429] - BSA and Azide free (ab250904)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13429] to S6K1 - BSA and Azide free
  • Suitable for: IHC-P, ICC, Flow Cyt (Intra), WB
  • Reacts with: Mouse, Rat, Human

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Overview

  • Product name

    Anti-S6K1 antibody [EPR13429] - BSA and Azide free
    See all S6K1 primary antibodies
  • Description

    Rabbit monoclonal [EPR13429] to S6K1 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, ICC, Flow Cyt (Intra), WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab250904 is the carrier-free version of ab186753.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Clonality

    Monoclonal
  • Clone number

    EPR13429
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Cell Cycle
    • Cell Cycle Inhibitors
    • Rb
    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • DNA Damage & Repair
    • DNA Damage Response
    • DNA Damage Recognition
    • Signal Transduction
    • Protein Phosphorylation
    • Ser / Thr Kinases
    • S6K Family
    • Metabolism
    • Types of disease
    • Obesity

Images

  • Western blot - Anti-S6K1 antibody [EPR13429] - BSA and Azide free (ab250904)
    Western blot - Anti-S6K1 antibody [EPR13429] - BSA and Azide free (ab250904)
    All lanes : Anti-S6K1 antibody [EPR13429] (ab186753) at 1/1000 dilution

    Lane 1 : 293 cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : HepG2 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 59 kDa
    Observed band size: 70 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab186753, the same antibody clone in a different buffer formulation.

  • Western blot - Anti-S6K1 antibody [EPR13429] - BSA and Azide free (ab250904)
    Western blot - Anti-S6K1 antibody [EPR13429] - BSA and Azide free (ab250904)
    All lanes : Anti-S6K1 antibody [EPR13429] (ab186753) at 1/1000 dilution

    Lane 1 : C6 cell lysate
    Lane 2 : RAW 264.7 cell lysate
    Lane 3 : PC-12 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

    Predicted band size: 59 kDa
    Observed band size: 70 kDa why is the actual band size different from the predicted?



    This data was developed using ab186753, the same antibody clone in a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S6K1 antibody [EPR13429] - BSA and Azide free (ab250904)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S6K1 antibody [EPR13429] - BSA and Azide free (ab250904)
    This data was developed using ab186753, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling S6K1 with ab186753 at 1/50 dilution, followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin. Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
  • Immunocytochemistry - Anti-S6K1 antibody [EPR13429] - BSA and Azide free (ab250904)
    Immunocytochemistry - Anti-S6K1 antibody [EPR13429] - BSA and Azide free (ab250904)
    This data was developed using ab186753, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 4% paraformaldehyde-fixed HeLa cells labeling S6K1 with ab186753 at 1/100 dilution, followed by Goat anti rabbit IgG (Alexa Fluor® 488) secondary antibody at 1/200 dilution. Counter stained with Dapi (blue).
  • Immunocytochemistry - Anti-S6K1 antibody [EPR13429] - BSA and Azide free (ab250904)
    Immunocytochemistry - Anti-S6K1 antibody [EPR13429] - BSA and Azide free (ab250904)
    This data was developed using ab186753, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 4% paraformaldehyde-fixed HepG2 cells labeling S6K1 with ab186753 at 1/100 dilution, followed by Goat anti rabbit IgG (Alexa Fluor® 488) secondary antibody at 1/200 dilution. Counter stained with Dapi (blue).
  • Flow Cytometry - Anti-S6K1 antibody [EPR13429] - BSA and Azide free (ab250904)
    Flow Cytometry - Anti-S6K1 antibody [EPR13429] - BSA and Azide free (ab250904)
    This data was developed using ab186753, the same antibody clone in a different buffer formulation.Flow cytometric analysis of 2% paraformaldehyde-fixed HeLa cells labeling P70 S6 Kinase alpha with ab186753 at 1/500 dilution (red) compared to a Rabbit monoclonal IgG isotype control (green), followed by Goat anti rabbit IgG (FITC) secondary antibody at 1/150 dilution.
  • Anti-S6K1 antibody [EPR13429] - BSA and Azide free (ab250904)
    Anti-S6K1 antibody [EPR13429] - BSA and Azide free (ab250904)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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