Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Pumilio 1 antibody [EPR3795] - BSA and Azide free (ab247584)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3795] to Pumilio 1 - BSA and Azide free
  • Suitable for: ICC, Flow Cyt, IHC-P, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Pumilio 1 antibody [EPR3795] - BSA and Azide free
    See all Pumilio 1 primary antibodies

  • Description

    Rabbit monoclonal [EPR3795] to Pumilio 1 - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    This antibody is predicted to not cross react with Pumilio 2.

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab247584 is the carrier-free version of ab92545 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab247584 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

     

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pumilio 1 antibody [EPR3795] - BSA and Azide free (ab247584)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pumilio 1 antibody [EPR3795] - BSA and Azide free (ab247584)

    This data was developed using ab92545, the same antibody clone in a different buffer formulation.

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human thyroid carcinoma tissue sections labeling Pumilio 1 with Purified ab92545 at 1:100 dilution (1.18 μg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.
  • Flow Cytometry - Anti-Pumilio 1 antibody [EPR3795] - BSA and Azide free (ab247584)
    Flow Cytometry - Anti-Pumilio 1 antibody [EPR3795] - BSA and Azide free (ab247584)

    This data was developed using ab92545, the same antibody clone in a different buffer formulation.

    Flow Cytometry analysis of HEK-293 (Human embryonic kidney epithelial cell) cells labeling Pumilio 1 with purified ab92545 at 1:20 dilution (10 ug/ml) (red). Cells were fixed with 4% Paraformaldehyde and permeabilized with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488) secondary antibody was used at 1:2000 dilution. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
  • Immunocytochemistry - Anti-Pumilio 1 antibody [EPR3795] - BSA and Azide free (ab247584)
    Immunocytochemistry - Anti-Pumilio 1 antibody [EPR3795] - BSA and Azide free (ab247584)

    This data was developed using ab92545, the same antibody clone in a different buffer formulation.

    Immunocytochemistry/ Immunofluorescence analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling Pumilio 1 with purified ab92545 at 1:500 dilution (0.2μg/ml). Cells were fixed in 100% Methanol. ab150077 Goat anti rabbit IgG(Alexa Fluor® 488) was used as the secondary antibody at 1:1000 dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
  • Western blot - Anti-Pumilio 1 antibody [EPR3795] - BSA and Azide free (ab247584)
    Western blot - Anti-Pumilio 1 antibody [EPR3795] - BSA and Azide free (ab247584)
    All lanes : Anti-Pumilio 1 antibody [EPR3795] (ab92545) at 1/1000 dilution (unpurified)

    Lane 1 : HeLa cell lysate
    Lane 2 : 293T cell lysates

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Standard HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 126 kDa



    This data was developed using ab92545, the same antibody clone in a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pumilio 1 antibody [EPR3795] - BSA and Azide free (ab247584)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pumilio 1 antibody [EPR3795] - BSA and Azide free (ab247584)

    This data was developed using ab92545, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of Pumilio 1 in paraffin embedded Human fetal kidney tissue using an unpurified ab92545 at a 1/100 dilution.
  • Western blot - Anti-Pumilio 1 antibody [EPR3795] - BSA and Azide free (ab247584)
    Western blot - Anti-Pumilio 1 antibody [EPR3795] - BSA and Azide free (ab247584)
    All lanes : Anti-Pumilio 1 antibody [EPR3795] (ab92545) at 1/1000 dilution (purified)

    Lane 1 : Mouse cerebellum tissue lysate
    Lane 2 : Rat brain tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 126 kDa



    This data was developed using ab92545, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-Pumilio 1 antibody [EPR3795] - BSA and Azide free (ab247584)
    Western blot - Anti-Pumilio 1 antibody [EPR3795] - BSA and Azide free (ab247584)
    All lanes : Anti-Pumilio 1 antibody [EPR3795] (ab92545) at 1/5000 dilution (purified)

    Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
    Lane 2 : HEK-293 (Human embryonic kidney epithelial cell) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 126 kDa



    This data was developed using ab92545, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Immunocytochemistry - Anti-Pumilio 1 antibody [EPR3795] - BSA and Azide free (ab247584)
    Immunocytochemistry - Anti-Pumilio 1 antibody [EPR3795] - BSA and Azide free (ab247584)
    This data was developed using ab92545, the same antibody clone in a different buffer formulation.Immunofluorescent staining of Pumilio 1 in HeLa cells using an unpurified ab92545 at a 1/100 dilution.
  • Flow Cytometry - Anti-Pumilio 1 antibody [EPR3795] - BSA and Azide free (ab247584)
    Flow Cytometry - Anti-Pumilio 1 antibody [EPR3795] - BSA and Azide free (ab247584)
    This data was developed using ab92545, the same antibody clone in a different buffer formulation.Overlay histogram showing HEK293 cells stained with an unpurified ab92545 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab92545, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1 µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HEK293 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

  • Anti-Pumilio 1 antibody [EPR3795] - BSA and Azide free (ab247584)
    Anti-Pumilio 1 antibody [EPR3795] - BSA and Azide free (ab247584)

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