Anti-BHMT antibody [EPR20076] - BSA and Azide free (ab223145)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR20076] to BHMT - BSA and Azide free
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-BHMT antibody [EPR20076] - BSA and Azide free
See all BHMT primary antibodies -
Description
Rabbit monoclonal [EPR20076] to BHMT - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Human BHMT1 recombinant protein; Human fetal kidney and fetal liver lysates; Mouse and rat kidney and liver lysates.
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General notes
Ab223145 is the carrier-free version of ab207765. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab223145 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR20076 -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-BHMT antibody [EPR20076] (ab207765) at 1/50000 dilution
Lane 1 : Human BHMT1 recombinant protein
Lane 2 : Human BHMT2 recombinant protein
Lysates/proteins at 0.02 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Predicted band size: 45 kDa
Observed band size: 45 kDa
Exposure time: 1 secondThis data was developed using ab207765, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
Human BHMT1 and BHMT2 recombinant proteins contain aa11-314 and aa1-363 respectively, and both are with a His-tag.
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All lanes : Anti-BHMT antibody [EPR20076] (ab207765) at 1/10000 dilution
Lane 1 : Human fetal kidney tissue lysate
Lane 2 : Human brain lysate
Lane 3 : Human lung lysate
Lane 4 : Human spleen lysate
Lane 5 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution
Predicted band size: 45 kDa
Observed band size: 45 kDa
Exposure time: 1 secondThis data was developed using ab207765, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
BHMT1 is mainly expressed in liver and kidney according to the literature (PMID: 9281325).
Negative control: HepG2 (PMID: 22138536).
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Anti-BHMT antibody [EPR20076] (ab207765) at 1/5000 dilution + Human fetal liver lysate at 1 µg
Secondary
Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/100000 dilution
Predicted band size: 45 kDa
Observed band size: 45 kDa
Exposure time: 5 secondsThis data was developed using ab207765, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
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Lane 1 : Anti-BHMT antibody [EPR20076] (ab207765) at 1/1000 dilution
Lane 2 : Anti-BHMT antibody [EPR20076] (ab207765) at 1/5000 dilution
Lanes 3-4 : Anti-BHMT antibody [EPR20076] (ab207765) at 1/20000 dilution
Lane 1 : Rat kidney lysate at 10 µg
Lane 2 : Mouse kidney lysate at 10 µg
Lane 3 : Mouse liver lysate at 0.5 µg
Lane 4 : Rat liver lysate at 0.5 µg
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 45 kDa
Observed band size: 45 kDaThis data was developed using ab207765, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
Exposure time: Lanes 1, 3-4: 1 second; Lane 2: 3 minutes.
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