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Signal Transduction Metabolism Amino Acids

Anti-BHMT antibody [EPR20076] - BSA and Azide free (ab223145)

Price and availability

526 012 ₸

Availability

Order now and get it on Wednesday March 03, 2021

Anti-BHMT antibody [EPR20076] - BSA and Azide free (ab223145)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR20076] to BHMT - BSA and Azide free
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Human

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Overview

  • Product name

    Anti-BHMT antibody [EPR20076] - BSA and Azide free
    See all BHMT primary antibodies
  • Description

    Rabbit monoclonal [EPR20076] to BHMT - BSA and Azide free
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data
  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human BHMT1 recombinant protein; Human fetal kidney and fetal liver lysates; Mouse and rat kidney and liver lysates.
  • General notes

    Ab223145 is the carrier-free version of ab207765. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab223145 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR20076
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Metabolism
    • Amino Acids
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Amino acid metabolism

Images

  • Western blot - Anti-BHMT antibody [EPR20076] - BSA and Azide free (ab223145)
    Western blot - Anti-BHMT antibody [EPR20076] - BSA and Azide free (ab223145)
    All lanes : Anti-BHMT antibody [EPR20076] (ab207765) at 1/50000 dilution

    Lane 1 : Human BHMT1 recombinant protein
    Lane 2 : Human BHMT2 recombinant protein

    Lysates/proteins at 0.02 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

    Predicted band size: 45 kDa
    Observed band size: 45 kDa


    Exposure time: 1 second


    This data was developed using ab207765, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Human BHMT1 and BHMT2 recombinant proteins contain aa11-314 and aa1-363 respectively, and both are with a His-tag.

  • Western blot - Anti-BHMT antibody [EPR20076] - BSA and Azide free (ab223145)
    Western blot - Anti-BHMT antibody [EPR20076] - BSA and Azide free (ab223145)
    All lanes : Anti-BHMT antibody [EPR20076] (ab207765) at 1/10000 dilution

    Lane 1 : Human fetal kidney tissue lysate
    Lane 2 : Human brain lysate
    Lane 3 : Human lung lysate
    Lane 4 : Human spleen lysate
    Lane 5 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

    Predicted band size: 45 kDa
    Observed band size: 45 kDa


    Exposure time: 1 second


    This data was developed using ab207765, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    BHMT1 is mainly expressed in liver and kidney according to the literature (PMID: 9281325).

    Negative control: HepG2 (PMID: 22138536).

  • Western blot - Anti-BHMT antibody [EPR20076] - BSA and Azide free (ab223145)
    Western blot - Anti-BHMT antibody [EPR20076] - BSA and Azide free (ab223145)
    Anti-BHMT antibody [EPR20076] (ab207765) at 1/5000 dilution + Human fetal liver lysate at 1 µg

    Secondary
    Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/100000 dilution

    Predicted band size: 45 kDa
    Observed band size: 45 kDa


    Exposure time: 5 seconds


    This data was developed using ab207765, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-BHMT antibody [EPR20076] - BSA and Azide free (ab223145)
    Western blot - Anti-BHMT antibody [EPR20076] - BSA and Azide free (ab223145)
    Lane 1 : Anti-BHMT antibody [EPR20076] (ab207765) at 1/1000 dilution
    Lane 2 : Anti-BHMT antibody [EPR20076] (ab207765) at 1/5000 dilution
    Lanes 3-4 : Anti-BHMT antibody [EPR20076] (ab207765) at 1/20000 dilution

    Lane 1 : Rat kidney lysate at 10 µg
    Lane 2 : Mouse kidney lysate at 10 µg
    Lane 3 : Mouse liver lysate at 0.5 µg
    Lane 4 : Rat liver lysate at 0.5 µg

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 45 kDa
    Observed band size: 45 kDa



    This data was developed using ab207765, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure time: Lanes 1, 3-4: 1 second; Lane 2: 3 minutes.

  • Anti-BHMT antibody [EPR20076] - BSA and Azide free (ab223145)
    Anti-BHMT antibody [EPR20076] - BSA and Azide free (ab223145)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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