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Signal Transduction Metabolism Amino Acids

Anti-BHMT antibody [EPR20076] (ab207765)

Price and availability

284 784 ₸

Availability

Order now and get it on Tuesday March 09, 2021

Anti-BHMT antibody [EPR20076] (ab207765)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR20076] to BHMT
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Human, Recombinant fragment

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Overview

  • Product name

    Anti-BHMT antibody [EPR20076]
    See all BHMT primary antibodies
  • Description

    Rabbit monoclonal [EPR20076] to BHMT
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    WB
    Mouse
    Rat
    Human
    Recombinant fragment
    See all applications and species data
  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human BHMT1 recombinant protein; Human fetal kidney and fetal liver lysates; Mouse and rat kidney and liver lysates.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR20076
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Metabolism
    • Amino Acids
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Amino acid metabolism

Images

  • Western blot - Anti-BHMT antibody [EPR20076] (ab207765)
    Western blot - Anti-BHMT antibody [EPR20076] (ab207765)
    All lanes : Anti-BHMT antibody [EPR20076] (ab207765) at 1/50000 dilution

    Lane 1 : Human BHMT1 recombinant protein
    Lane 2 : Human BHMT2 recombinant protein

    Lysates/proteins at 0.02 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

    Predicted band size: 45 kDa
    Observed band size: 45 kDa


    Exposure time: 1 second


    Blocking/Dilution buffer: 5% NFDM/TBST.

    Human BHMT1 and BHMT2 recombinant proteins contain aa11-314 and aa1-363 respectively, and both are with a His-tag.

  • Western blot - Anti-BHMT antibody [EPR20076] (ab207765)
    Western blot - Anti-BHMT antibody [EPR20076] (ab207765)
    All lanes : Anti-BHMT antibody [EPR20076] (ab207765) at 1/10000 dilution

    Lane 1 : Human fetal kidney lysate
    Lane 2 : Human brain lysate
    Lane 3 : Human lung lysate
    Lane 4 : Human spleen lysate
    Lane 5 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

    Predicted band size: 45 kDa
    Observed band size: 45 kDa


    Exposure time: 1 second


    5% NFDM/TBST: Blocking and dilution buffer.

    BHMT1 is mainly expressed in liver and kidney according to the literature (PMID: 9281325). Negative control: HepG2 (PMID: 22138536).

  • Western blot - Anti-BHMT antibody [EPR20076] (ab207765)
    Western blot - Anti-BHMT antibody [EPR20076] (ab207765)
    Anti-BHMT antibody [EPR20076] (ab207765) at 1/5000 dilution + Human fetal liver lysate at 1 µg

    Secondary
    Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/100000 dilution

    Predicted band size: 45 kDa
    Observed band size: 45 kDa


    Exposure time: 5 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-BHMT antibody [EPR20076] (ab207765)
    Western blot - Anti-BHMT antibody [EPR20076] (ab207765)
    Lane 1 : Anti-BHMT antibody [EPR20076] (ab207765) at 1/1000 dilution
    Lane 2 : Anti-BHMT antibody [EPR20076] (ab207765) at 1/5000 dilution
    Lanes 3-4 : Anti-BHMT antibody [EPR20076] (ab207765) at 1/20000 dilution

    Lane 1 : Rat kidney lysate at 10 µg
    Lane 2 : Mouse kidney lysate at 10 µg
    Lane 3 : Mouse liver lysate at 0.5 µg
    Lane 4 : Rat liver lysate at 0.5 µg

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 45 kDa
    Observed band size: 45 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: Lane 1/3/4: 1 second; Lane 2: 3 minutes.

  • Anti-BHMT antibody [EPR20076] (ab207765)
    Anti-BHMT antibody [EPR20076] (ab207765)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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