Anti-BHMT antibody [EPR20076] (ab207765)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR20076] to BHMT
- Suitable for: WB
- Reacts with: Mouse, Rat, Human, Recombinant fragment
Overview
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Product name
Anti-BHMT antibody [EPR20076]
See all BHMT primary antibodies -
Description
Rabbit monoclonal [EPR20076] to BHMT -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB MouseRatHumanRecombinant fragment -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Human BHMT1 recombinant protein; Human fetal kidney and fetal liver lysates; Mouse and rat kidney and liver lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR20076 -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-BHMT antibody [EPR20076] (ab207765) at 1/50000 dilution
Lane 1 : Human BHMT1 recombinant protein
Lane 2 : Human BHMT2 recombinant protein
Lysates/proteins at 0.02 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Predicted band size: 45 kDa
Observed band size: 45 kDa
Exposure time: 1 secondBlocking/Dilution buffer: 5% NFDM/TBST.
Human BHMT1 and BHMT2 recombinant proteins contain aa11-314 and aa1-363 respectively, and both are with a His-tag.
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All lanes : Anti-BHMT antibody [EPR20076] (ab207765) at 1/10000 dilution
Lane 1 : Human fetal kidney lysate
Lane 2 : Human brain lysate
Lane 3 : Human lung lysate
Lane 4 : Human spleen lysate
Lane 5 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution
Predicted band size: 45 kDa
Observed band size: 45 kDa
Exposure time: 1 second5% NFDM/TBST: Blocking and dilution buffer.
BHMT1 is mainly expressed in liver and kidney according to the literature (PMID: 9281325). Negative control: HepG2 (PMID: 22138536).
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Anti-BHMT antibody [EPR20076] (ab207765) at 1/5000 dilution + Human fetal liver lysate at 1 µg
Secondary
Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/100000 dilution
Predicted band size: 45 kDa
Observed band size: 45 kDa
Exposure time: 5 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
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Lane 1 : Anti-BHMT antibody [EPR20076] (ab207765) at 1/1000 dilution
Lane 2 : Anti-BHMT antibody [EPR20076] (ab207765) at 1/5000 dilution
Lanes 3-4 : Anti-BHMT antibody [EPR20076] (ab207765) at 1/20000 dilution
Lane 1 : Rat kidney lysate at 10 µg
Lane 2 : Mouse kidney lysate at 10 µg
Lane 3 : Mouse liver lysate at 0.5 µg
Lane 4 : Rat liver lysate at 0.5 µg
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 45 kDa
Observed band size: 45 kDaBlocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: Lane 1/3/4: 1 second; Lane 2: 3 minutes.
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