Antibodies, Proteins, Kits and Reagents for Life Science

291 484 ₸ Product size

100 µl 291 484 ₸

Order now and get it on Wednesday March 10, 2021

Anti-BubR1 (phospho S670) antibody [EPR20109] (ab200062)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR20109] to BubR1 (phospho S670)
Suitable for: WB, ICC/IF
Reacts with: Human

Product name

Anti-BubR1 (phospho S670) antibody [EPR20109]
See all BubR1 primary antibodies
Description

Rabbit monoclonal [EPR20109] to BubR1 (phospho S670)
Host species

Rabbit
Tested Applications & Species

Application Species
ICC/IF
Human

WB
Human

See all applications and species data
Immunogen

This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: HeLa whole cell lysate treated with 0.5 µM nocodazole for 24 hours. ICC/IF: HeLa cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
ICC/IF	Human
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR20109
Isotype

IgG
Research areas

Western blot - Anti-BubR1 (phospho S670) antibody [EPR20109] (ab200062)

All lanes : Anti-BubR1 (phospho S670) antibody [EPR20109] (ab200062) at 1/5000 dilution

Lane 1 : Untreated HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate treated with 0.5 µM nocodazole for 24 hours
Lane 3 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate treated with 0.5 µM nocodazole for 24 hours, then with alkaline phosphatase for 1 hour

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 120 kDa

Exposure time: 10 seconds

Blocking/Dilution buffer: 5% NFDM/TBST.
Immunocytochemistry/ Immunofluorescence - Anti-BubR1 (phospho S670) antibody [EPR20109] (ab200062)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling BubR1 (phospho S670) with ab200062 at 1/500 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

Confocal image showing strong signal on mitotic phase of HeLa cells, the expression decreased after treatment with lambda protein phosphatase 31°C for 2h.

The nuclear counterstain is DAPI (blue).

Tubulin is detected with ab195889 (Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594)) at 1/200 dilution (red).

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor^® 488) (ab150077) at 1/1000 dilution.
Immunocytochemistry/ Immunofluorescence - Anti-BubR1 (phospho S670) antibody [EPR20109] (ab200062)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling BubR1 (phospho S670) with ab200062 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

Confocal image showing strong signal on mitotic phase of HeLa cells, the expression decreased after treatment with lambda protein phosphatase 31°C for 2h.

The nuclear counterstain is DAPI (blue).

Tubulin is detected with ab195889 (Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594)) at 1/200 dilution (red).

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor^® 488) (ab150077) at 1/1000 dilution.
Anti-BubR1 (phospho S670) antibody [EPR20109] (ab200062)

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