Antibodies, Proteins, Kits and Reagents for Life Science

291 484 ₸ Product size

100 µl 291 484 ₸

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Anti-BubR1 (phospho T680) antibody [EPR19958] (ab200061)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR19958] to BubR1 (phospho T680)
Suitable for: IP, Flow Cyt, ICC/IF, WB
Reacts with: Human

Product name

Anti-BubR1 (phospho T680) antibody [EPR19958]
See all BubR1 primary antibodies
Description

Rabbit monoclonal [EPR19958] to BubR1 (phospho T680)
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
IP	Human
WB	Human

See all applications and species data

Immunogen

This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: HeLa whole cell lysate treated with 0.5 µM nocodazole for 24 hours. ICC/IF: HeLa cells. Flow Cyt: HeLa (human cervix adenocarcinoma) treated with 0.5 ng/ml nocodazole for 24 hours. IP: HeLa whole cell lysate treated with 0.5 µM nocodazole for 24 hours.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR19958
Isotype

IgG
Research areas

Immunocytochemistry/ Immunofluorescence - Anti-BubR1 (phospho T680) antibody [EPR19958] (ab200061)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling BubR1 (phospho T680) with ab200061 at 1/100 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

Confocal image showing positive staining on M phase HeLa cells, and LP treatment completely blocked the staining. (PMID:11792804).

The nuclear counter stain is DAPI (blue).

Tubulin is detected with ab195889 (Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594)) at 1/250 dilution (red).

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 at 1/1000 dilution.
Western blot - Anti-BubR1 (phospho T680) antibody [EPR19958] (ab200061)

All lanes : Anti-BubR1 (phospho T680) antibody [EPR19958] (ab200061) at 1/2000 dilution

Lane 1 : Untreated HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate treated with 0.5 µM nocodazole for 24 hours
Lane 3 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate treated with 0.5 µM nocodazole for 24 hours, then treated with FastAP Thermosensitive Alkaline Phosphatase for 1 hour

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 120 kDa
Observed band size: 120 kDa

Exposure time: 3 minutes

Blocking/Dilution buffer: 5% NFDM/TBST.
Flow Cytometry - Anti-BubR1 (phospho T680) antibody [EPR19958] (ab200061)

Flow cytometric analysis of 4% paraformaldehyde-fixed HeLa (Human epithelial cell line from cervix adenocarcinoma) cells, untreated or treated with 0.5 ng/ml nocodazole for 24 hours, labeling BubR1 (phospho T680) with ab200061 at 1/500 dilution compared with a Rabbit IgG,monoclonal [EPR25A]-Isotype control (ab172730) (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat anti Rabbit IgG (Alexa Fluor^® 488) at 1/2000 dilution was used as the secondary antibody.
Immunoprecipitation - Anti-BubR1 (phospho T680) antibody [EPR19958] (ab200061)

BubR1 (phospho T680) was immunoprecipitated from 0.35 mg of HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate treated with 0.5 μΜ nocodazole for 24 hours with ab200061 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab200061 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

Lane 1: HeLa whole cell lysate treated with 0.5 μΜ nocodazole for 24 hours 10µg (Input).

Lane 2: ab200061 IP in HeLa whole cell lysate treated with 0.5 μΜ nocodazole for 24 hours.

Lane 3: Rabbit IgG,monoclonal [EPR25A]-Isotype Control (ab172730) instead of ab200061 in HeLa whole cell lysate treated with 0.5 μΜ nocodazole for 24 hours.

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 3 seconds.
Anti-BubR1 (phospho T680) antibody [EPR19958] (ab200061)

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