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Anti-BubR1 (phospho T680) antibody [EPR19958] (ab200061)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR19958] to BubR1 (phospho T680)
  • Suitable for: IP, Flow Cyt, ICC/IF, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-BubR1 (phospho T680) antibody [EPR19958]
    See all BubR1 primary antibodies

  • Description

    Rabbit monoclonal [EPR19958] to BubR1 (phospho T680)

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa whole cell lysate treated with 0.5 µM nocodazole for 24 hours. ICC/IF: HeLa cells. Flow Cyt: HeLa (human cervix adenocarcinoma) treated with 0.5 ng/ml nocodazole for 24 hours. IP: HeLa whole cell lysate treated with 0.5 µM nocodazole for 24 hours.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-BubR1 (phospho T680) antibody [EPR19958] (ab200061)
    Immunocytochemistry/ Immunofluorescence - Anti-BubR1 (phospho T680) antibody [EPR19958] (ab200061)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling BubR1 (phospho T680) with ab200061 at 1/100 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

    Confocal image showing positive staining on M phase HeLa cells, and LP treatment completely blocked the staining. (PMID:11792804).

    The nuclear counter stain is DAPI (blue).

    Tubulin is detected with ab195889 (Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594)) at 1/250 dilution (red).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 at 1/1000 dilution.

  • Western blot - Anti-BubR1 (phospho T680) antibody [EPR19958] (ab200061)
    Western blot - Anti-BubR1 (phospho T680) antibody [EPR19958] (ab200061)
    All lanes : Anti-BubR1 (phospho T680) antibody [EPR19958] (ab200061) at 1/2000 dilution

    Lane 1 : Untreated HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 2 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate treated with 0.5 µM nocodazole for 24 hours
    Lane 3 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate treated with 0.5 µM nocodazole for 24 hours, then treated with FastAP Thermosensitive Alkaline Phosphatase for 1 hour

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 120 kDa
    Observed band size: 120 kDa


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

     

  • Flow Cytometry - Anti-BubR1 (phospho T680) antibody [EPR19958] (ab200061)
    Flow Cytometry - Anti-BubR1 (phospho T680) antibody [EPR19958] (ab200061)

    Flow cytometric analysis of 4% paraformaldehyde-fixed HeLa (Human epithelial cell line from cervix adenocarcinoma) cells, untreated or treated with 0.5 ng/ml nocodazole for 24 hours, labeling BubR1 (phospho T680) with ab200061 at 1/500 dilution compared with a Rabbit IgG,monoclonal [EPR25A]-Isotype control (ab172730) (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat anti Rabbit IgG (Alexa Fluor® 488) at 1/2000 dilution was used as the secondary antibody.

  • Immunoprecipitation - Anti-BubR1 (phospho T680) antibody [EPR19958] (ab200061)
    Immunoprecipitation - Anti-BubR1 (phospho T680) antibody [EPR19958] (ab200061)

    BubR1 (phospho T680) was immunoprecipitated from 0.35 mg of HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate treated with 0.5 μΜ nocodazole for 24 hours with ab200061 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab200061 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

    Lane 1: HeLa whole cell lysate treated with 0.5 μΜ nocodazole for 24 hours 10µg (Input).

    Lane 2: ab200061 IP in HeLa whole cell lysate treated with 0.5 μΜ nocodazole for 24 hours.

    Lane 3: Rabbit IgG,monoclonal [EPR25A]-Isotype Control (ab172730) instead of ab200061 in HeLa whole cell lysate treated with 0.5 μΜ nocodazole for 24 hours.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 seconds.

  • Anti-BubR1 (phospho T680) antibody [EPR19958] (ab200061)
    Anti-BubR1 (phospho T680) antibody [EPR19958] (ab200061)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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