Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-SRAP antibody [EPR11776] (ab183725)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR11776] to SRAP
  • Suitable for: ICC/IF, Flow Cyt, IHC-P, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-SRAP antibody [EPR11776]
    See all SRAP primary antibodies

  • Description

    Rabbit monoclonal [EPR11776] to SRAP

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Human fetal liver and kidney lysates; Human ovarian carcinoma tissue; 293 and MCF7 cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-SRAP antibody [EPR11776] (ab183725)
    Western blot - Anti-SRAP antibody [EPR11776] (ab183725)
    Anti-SRAP antibody [EPR11776] (ab183725) at 1/2000 dilution + Human fetal liver lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 26 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SRAP antibody [EPR11776] (ab183725)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SRAP antibody [EPR11776] (ab183725)

    Immunohistochemical analysis of paraffin-embedded Human ovarian carcinoma tissue labeling SRAP with ab183725 at 1/100 dilution. The slide is counterstained with Hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-SRAP antibody [EPR11776] (ab183725)
    Immunocytochemistry/ Immunofluorescence - Anti-SRAP antibody [EPR11776] (ab183725)

    Immunofluorescence analysis of 4% paraformaldehyde-fixed MCF7 cells labeling SRAP with ab183725 at 1/500 dilution (red). Goat anti-rabbit IgG (Alexa Fluor® 555) at 1/200 dilution was used as the secondary antibody. The slide on the right is stained with Dapi (blue).

  • Western blot - Anti-SRAP antibody [EPR11776] (ab183725)
    Western blot - Anti-SRAP antibody [EPR11776] (ab183725)
    Anti-SRAP antibody [EPR11776] (ab183725) at 1/10000 dilution + Human fetal kidney lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated

    Predicted band size: 26 kDa

  • Flow Cytometry - Anti-SRAP antibody [EPR11776] (ab183725)
    Flow Cytometry - Anti-SRAP antibody [EPR11776] (ab183725)

    Flow cytometric analysis of 2% paraformaldehyde-fixed 293 cells labeling SRAP with ab183725 at 1/180 dilution. Goat anti-rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody. Rabbit monoclonal IgG was used as the isotype control.

  • Anti-SRAP antibody [EPR11776] (ab183725)
    Anti-SRAP antibody [EPR11776] (ab183725)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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