Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Glutamate Receptor 1 (AMPA subtype) (phospho S845) antibody [EPR2148] - BSA and Azide free (ab208790)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR2148] to Glutamate Receptor 1 (AMPA subtype) (phospho S845) - BSA and Azide free
  • Suitable for: WB, Dot blot
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Glutamate Receptor 1 (AMPA subtype) (phospho S845) antibody [EPR2148] - BSA and Azide free
    See all Glutamate Receptor 1 (AMPA subtype) primary antibodies

  • Description

    Rabbit monoclonal [EPR2148] to Glutamate Receptor 1 (AMPA subtype) (phospho S845) - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Rat
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human cerebellum lysate.

  • General notes

    Ab208790 is the carrier-free version of ab76321. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab208790 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Dot Blot - Anti-Glutamate Receptor 1 (AMPA subtype) (phospho S845) antibody [EPR2148] - BSA and Azide free (ab208790)
    Dot Blot - Anti-Glutamate Receptor 1 (AMPA subtype) (phospho S845) antibody [EPR2148] - BSA and Azide free (ab208790)

    Dot blot analysis of Glutamate Receptor 1 (AMPA subtype) labelling Glutamate Receptor 1 (AMPA subtype) (phospho S845) with ab76321 at 1/1000 in 5% NFDM/TBST. Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) (1/100,000) was used as the secondary antibody. Exposed for 3 minutes.

    Lane 1: Glutamate Receptor 1 (AMPA subtype) (pS845) phospho peptide  

    Lane 2: Glutamate Receptor 1 (AMPA subtype) non-phospho peptide

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab76321).

  • Western blot - Anti-Glutamate Receptor 1 (AMPA subtype) (phospho S845) antibody [EPR2148] - BSA and Azide free (ab208790)
    Western blot - Anti-Glutamate Receptor 1 (AMPA subtype) (phospho S845) antibody [EPR2148] - BSA and Azide free (ab208790)
    All lanes : Anti-Glutamate Receptor 1 (AMPA subtype) (phospho S845) antibody [EPR2148] (ab76321) at 1/1000 dilution

    Lane 1 : Human cerebellum whole cell lysates
    Lane 2 : Human cerebellum whole cell lysates. The membrane was incubated with phosphatase.

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 102 kDa
    Observed band size: 106 kDa
    why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    Blocked with 5% NFDM/TBST.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab76321).

  • Anti-Glutamate Receptor 1 (AMPA subtype) (phospho S845) antibody [EPR2148] - BSA and Azide free (ab208790)
    Anti-Glutamate Receptor 1 (AMPA subtype) (phospho S845) antibody [EPR2148] - BSA and Azide free (ab208790)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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