Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CD30 antibody [BLR055F] (ab252561)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [BLR055F] to CD30
  • Suitable for: WB, IHC-P, IP, ICC
  • Reacts with: Human

Overview

  • Product name

    Anti-CD30 antibody [BLR055F]
    See all CD30 primary antibodies

  • Description

    Rabbit monoclonal [BLR055F] to CD30

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC
    Human
    IHC-P
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment corresponding to Human CD30 aa 1-400. NP_001234.3. Extracellular domain.
    Database link: P28908

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • WB: MJ, SR and C5/MJ whole cell lysates. IP: MJ whole cell lysate. IHC-P: Human tonsil tissue. ICC: SR cells.

  • General notes

    This product is sold under License from Bethyl Laboratories, Inc

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD30 antibody [BLR055F] (ab252561)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD30 antibody [BLR055F] (ab252561)

    Formalin-fixed, paraffin-embedded human tonsil tissue stained for CD30 using ab252561 at 1/250 dilution in immunohistochemical analysis. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: DAB staining.

  • Western blot - Anti-CD30 antibody [BLR055F] (ab252561)
    Western blot - Anti-CD30 antibody [BLR055F] (ab252561)
    All lanes : Anti-CD30 antibody [BLR055F] (ab252561) at 1/1000 dilution

    Lane 1 : MJ (human cutaneous T cell lymphoma cell line) whole cell lysate
    Lane 2 : SR (human large cell immunoblastic lymphoma cell line) whole cell lysate
    Lane 3 : C5/MJ (human leukemia T cell lymphocyte cell line) whole cell lysate
    Lane 4 : GaMG (human cell line) whole cell lysate
    Lane 5 : U-2 OS (human bone osteosarcoma epithelial cell line) whole cell lysate
    Lane 6 : A549 (human lung carcinoma cell line) whole cell lysate

    Lysates/proteins at 50 µg per lane.

    Secondary
    All lanes : HRP-conjugated goat anti-rabbit IgG

    Developed using the ECL technique.

    Predicted band size: 64 kDa


    Exposure time: 30 seconds


    Lysates prepared in NETN buffer.

  • Immunocytochemistry - Anti-CD30 antibody [BLR055F] (ab252561)
    Immunocytochemistry - Anti-CD30 antibody [BLR055F] (ab252561)

    Formalin-fixed, paraffin-embedded sections of SR (human large cell immunoblastic lymphoma cell line) cells stained for CD30 using ab252561 at 1/250 dilution in immunohistochemical analysis. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: DAB staining.

  • Immunoprecipitation - Anti-CD30 antibody [BLR055F] (ab252561)
    Immunoprecipitation - Anti-CD30 antibody [BLR055F] (ab252561)

    CD30 was immunoprecipitated from MJ (human cutaneous T cell lymphoma cell line) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab252561 at 5 μL per reaction. Western blot was performed from the immunoprecipitate using ab252561 at 1/1000 dilution.

    Lane 1: ab252561 IP in MJ whole cell lysate.
    Lane 2: Control IgG IP in MJ whole cell lysate.

    Detection: Chemiluminescence with exposure time of 30 seconds.

    Lysates prepared in NETN buffer.

  • Anti-CD30 antibody [BLR055F] (ab252561)
    Anti-CD30 antibody [BLR055F] (ab252561)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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