Antibodies, Proteins, Kits and Reagents for Life Science

304 886 ₸ Product size

100 µl 304 886 ₸

Order now and get it on Tuesday March 02, 2021

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [BLR055F] to CD30
Suitable for: WB, IHC-P, IP, ICC
Reacts with: Human

Product name

Anti-CD30 antibody [BLR055F]
See all CD30 primary antibodies
Description

Rabbit monoclonal [BLR055F] to CD30
Host species

Rabbit

Tested Applications & Species

Application	Species
ICC	Human
IHC-P	Human
IP	Human
WB	Human

See all applications and species data

Immunogen

Recombinant fragment corresponding to Human CD30 aa 1-400. NP_001234.3. Extracellular domain.
Database link: P28908

Run BLAST with Run BLAST with
Positive control
- WB: MJ, SR and C5/MJ whole cell lysates. IP: MJ whole cell lysate. IHC-P: Human tonsil tissue. ICC: SR cells.
General notes

This product is sold under License from Bethyl Laboratories, Inc

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.8
Preservative: 0.09% Sodium azide
Constituents: 99% Borate buffered saline, 0.1% BSA
Concentration information loading...
Purification notes

Purified from cell culture supernatant.
Clonality

Monoclonal
Clone number

BLR055F
Isotype

IgG
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD30 antibody [BLR055F] (ab252561)

Formalin-fixed, paraffin-embedded human tonsil tissue stained for CD30 using ab252561 at 1/250 dilution in immunohistochemical analysis. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: DAB staining.
Western blot - Anti-CD30 antibody [BLR055F] (ab252561)

All lanes : Anti-CD30 antibody [BLR055F] (ab252561) at 1/1000 dilution

Lane 1 : MJ (human cutaneous T cell lymphoma cell line) whole cell lysate
Lane 2 : SR (human large cell immunoblastic lymphoma cell line) whole cell lysate
Lane 3 : C5/MJ (human leukemia T cell lymphocyte cell line) whole cell lysate
Lane 4 : GaMG (human cell line) whole cell lysate
Lane 5 : U-2 OS (human bone osteosarcoma epithelial cell line) whole cell lysate
Lane 6 : A549 (human lung carcinoma cell line) whole cell lysate

Lysates/proteins at 50 µg per lane.

Secondary
All lanes : HRP-conjugated goat anti-rabbit IgG

Developed using the ECL technique.

Predicted band size: 64 kDa

Exposure time: 30 seconds

Lysates prepared in NETN buffer.
Immunocytochemistry - Anti-CD30 antibody [BLR055F] (ab252561)

Formalin-fixed, paraffin-embedded sections of SR (human large cell immunoblastic lymphoma cell line) cells stained for CD30 using ab252561 at 1/250 dilution in immunohistochemical analysis. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: DAB staining.
Immunoprecipitation - Anti-CD30 antibody [BLR055F] (ab252561)

CD30 was immunoprecipitated from MJ (human cutaneous T cell lymphoma cell line) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab252561 at 5 μL per reaction. Western blot was performed from the immunoprecipitate using ab252561 at 1/1000 dilution.

Lane 1: ab252561 IP in MJ whole cell lysate.
Lane 2: Control IgG IP in MJ whole cell lysate.

Detection: Chemiluminescence with exposure time of 30 seconds.

Lysates prepared in NETN buffer.
Anti-CD30 antibody [BLR055F] (ab252561)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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