Anti-Rab3D antibody [EPR8106] (ab128997)
![Anti-Rab3D antibody [EPR8106] (ab128997)](https://www.abcam.com/ps/products/128/ab128997/Images/ab128997-142885-anti-rab3d-antibody-epr8106-immunohistochemistry.jpg "Anti-Rab3D antibody [EPR8106] (ab128997)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8106] to Rab3D
- Suitable for: WB, IHC-P
- Reacts with: Human
Overview
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Product name
Anti-Rab3D antibody [EPR8106]
See all Rab3D primary antibodies -
Description
Rabbit monoclonal [EPR8106] to Rab3D -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
Synthetic peptide within Human Rab3D aa 1-100 (N terminal). The exact sequence is proprietary.
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Positive control
- BxPC-3, T.T, A549, or HT-29 lysate, Human clear cell carcinoma tissue, Human stomach tissue.
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 3.36 x 10 -10 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR8106 -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rab3D antibody [EPR8106] (ab128997)
ab128997, at a 1/250 dilution, staining Human Rab3D in clear cell carcinoma, using Immunohistochemistry, Formalin/PFA-fixed paraffin-emebedded tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Western blot - Anti-Rab3D antibody [EPR8106] (ab128997)](https://www.abcam.com/ps/products/128/ab128997/Images/ab128997-142886-anti-rab3d-antibody-epr8106-western-blot.jpg)
Western blot - Anti-Rab3D antibody [EPR8106] (ab128997)All lanes : Anti-Rab3D antibody [EPR8106] (ab128997) at 1/10000 dilution
Lane 1 : BxPC-3 cell lysate
Lane 2 : T.T cell lysate
Lane 3 : A549 cell lysate
Lane 4 : HT-29 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Standard HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 25 kDa
Observed band size: 25 kDa
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rab3D antibody [EPR8106] (ab128997)](https://www.abcam.com/ps/products/128/ab128997/Images/ab128997-142884-anti-rab3d-antibody-epr8106-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rab3D antibody [EPR8106] (ab128997)ab128997, at a 1/250 dilution, staining Human Rab3D in stomach, using Immunohistochemistry, Formalin/PFA-fixed paraffin-emebedded tissue
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![OI-RD Scanning - Anti-Rab3D antibody [EPR8106] (ab128997)](https://www.abcam.com/ps/products/128/ab128997/Images/ab128997-142883-anti-rab3d-antibody-epr8106.jpg)
OI-RD Scanning - Anti-Rab3D antibody [EPR8106] (ab128997) -
![Anti-Rab3D antibody [EPR8106] (ab128997)](https://www.abcam.com/ps/products/128/ab128997/Images/ab128997-5-benefits-of-recombinant-antibodies.png)
Anti-Rab3D antibody [EPR8106] (ab128997)
