Anti-FGFR1 alpha antibody [M2F12] (ab829)
![Anti-FGFR1 alpha antibody [M2F12] (ab829)](https://www.abcam.com/ps/products/0/ab829/Images/ab829-3779-anti-fgfr1-alpha-antibody-m2f12-immunofluorescence.jpg "Anti-FGFR1 alpha antibody [M2F12] (ab829)")
Key features and details
- Mouse monoclonal [M2F12] to FGFR1 alpha
- Suitable for: ICC/IF
- Reacts with: Human
- Isotype: IgG2a
Overview
-
Product name
Anti-FGFR1 alpha antibody [M2F12]
See all FGFR1 alpha primary antibodies -
Description
Mouse monoclonal [M2F12] to FGFR1 alpha -
Host species
Mouse -
Specificity
Reacts with alpha isoform of FRFR1. -
Tested applications
Suitable for: ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant human ectodomain of FGFr1a expressed in E. coli beginning with pro23; antigen contained NH2-terminal gly-ser-pro-gly-ile and COOH-terminal glu-phe sequences.
-
Epitope
NH2-terminus of unique NH2-terminal Ig loop of FGFr1. Epitope is within the sequence between glu30 and ala74 of FGFr1a. -
Positive control
- IHC: Rat prostate tissue.
-
General notes
This product was changed from ascites to tissue culture supernatant on 19/12/2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions please do not hesitate to contact our scientific support team.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.40
Constituent: PBS -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purified from TCS -
Clonality
Monoclonal -
Clone number
M2F12 -
Isotype
IgG2a -
Research areas
Images
-
Immunocytochemistry/ Immunofluorescence - Anti-FGFR1 alpha antibody [M2F12] (ab829)
ICC/IF image of ab829 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab829, 10µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
This image was generated using the ascites version of the product.
