Anti-FGFR1 alpha (phospho Y654) antibody [EP843(2)] (ab134043)
Key features and details
- Rabbit monoclonal [EP843(2)] to FGFR1 alpha (phospho Y654)
- Suitable for: WB
- Isotype: IgG
Overview
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Product name
Anti-FGFR1 alpha (phospho Y654) antibody [EP843(2)]
See all FGFR1 alpha primary antibodies -
Description
Rabbit monoclonal [EP843(2)] to FGFR1 alpha (phospho Y654) -
Host species
Rabbit -
Specificity
Only detects FGFR1 alpha when phosphorylated at Tyrosine 654 -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,ICC,IHC-P or IP -
Species reactivity
Predicted to work with: Mouse -
Immunogen
A phospho specific peptide corresponding to residues surrounding Tyrosine 654 of Human FGFR1 alpha.
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Positive control
- Constitutively active FGFR1
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General notes
Human, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 1.60 x 10 -10 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EP843(2) -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-FGFR1 alpha (phospho Y654) antibody [EP843(2)] (ab134043) at 1/1000 dilution
Lane 1 : Control
Lane 2 : Constitutively active FGFR1 alpha
Lane 3 : Kinase dead lysates from Xenopus oocytes
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-
rabbit at 1/2000 dilution
Predicted band size: 130 kDa