Anti-Cytokeratin 18 antibody - C-terminal (ab189444)
Key features and details
- Rabbit polyclonal to Cytokeratin 18 - C-terminal
- Suitable for: ICC/IF, WB, IHC-P, Flow Cyt
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Cytokeratin 18 antibody - C-terminal
See all Cytokeratin 18 primary antibodies -
Description
Rabbit polyclonal to Cytokeratin 18 - C-terminal -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WB, IHC-P, Flow Cytmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide corresponding to Human Cytokeratin 18 (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH).
Database link: P05783 -
Positive control
- Human prostate, prostate carcinoma and colon carcinoma tissue. K562, NCI-H460 and mouse stomach cell lysates. HeLa and WiDr cells.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituent: 99% PBS -
Concentration information loading...
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Purity
Ammonium Sulphate Precipitation -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-Cytokeratin 18 antibody - C-terminal (ab189444) at 1/1000 dilution
Lane 1 : K562 cell lysate
Lane 2 : NCI-H460 cell lysate
Lane 3 : Mouse stomach tissue lysate
Lysates/proteins at 35 µg per lane.
Predicted band size: 48 kDa
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Immunohistochemistry analysis of formalin-fixed, paraffin-embedded human colon carcinoma tissue labeling Cytokeratin 18 with ab189444 at 5 µg/ml. ab189444 was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
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Immunohistochemistry analysis of formalin-fixed, paraffin-embedded human prostate carcinoma tissue labeling Cytokeratin 18 with ab189444 at 5 µg/ml.
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Immunohistochemistry analysis of formalin-fixed, paraffin-embedded human prostate tissue labeling Cytokeratin 18 with ab189444 at 5 µg/ml.
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Immunocytochemistry/ Immunofluorescence analysis of human prostate carcinoma tissue labeling Cytokeratin 18 with ab189444 at 1/10 dilution followed by Alexa Fluor 488-conjugated Goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
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Immunocytochemistry/ Immunofluorescence analysis of HeLa cells labeling Cytokeratin 18 with ab189444 at 1/10 dilution followed by Alexa Fluor 488-conjugated Goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).
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Flow cytometry analysis of WiDr cells using ab189444 at 1/10 dilution (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated Goat-anti-rabbit secondary antibodies were used for the analysis.