All lanes : Anti-C6orf211/ARMT1 antibody (ab241977) at 1/1000 dilution

Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate

Lysates/proteins at 50 µg per lane.

Developed using the ECL technique.

Predicted band size: 51 kDa


Exposure time: 30 seconds

Lysates prepared in NETN buffer.

C6orf211/ARMT1 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab241977 at 20 µl per reaction. Western blot was performed from the immunoprecipitate using ab241977 at 1/100 dilution.

Lane 1: ab241977 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG IP in HEK-293T whole cell lysate.

Detection: Chemiluminescence with exposure time of 30 seconds.

Lysates prepared in NETN buffer.