HRP Anti-COX IV antibody [20E8C12] (ab197920)
![HRP Anti-COX IV antibody [20E8C12] (ab197920)](https://www.abcam.com/ps/products/197/ab197920/Images/ab197920-238815-anti-cox-iv-antibody-20e8c12-hrp-immunohistochemistry.jpg "HRP Anti-COX IV antibody [20E8C12] (ab197920)")
Key features and details
- HRP Mouse monoclonal [20E8C12] to COX IV
- Suitable for: WB, IHC-P
- Reacts with: Human
- Conjugation: HRP
- Isotype: IgG2a
Overview
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Product name
HRP Anti-COX IV antibody [20E8C12]
See all COX IV primary antibodies -
Description
HRP Mouse monoclonal [20E8C12] to COX IV -
Host species
Mouse -
Conjugation
HRP -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow, Drosophila melanogaster, Zebrafish
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Immunogen
Tissue, cells or virus corresponding to Cow COX IV. Purified mitochondrial Complex IV (Cow).
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Positive control
- WB: Human heart mitochondria lysate. IHC-P: Normal human colon tissue.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, PBS -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
20E8C12 -
Isotype
IgG2a -
Light chain type
kappa -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-COX IV antibody [20E8C12] (ab197920)
IHC image of COX IV staining in a section of formalin-fixed paraffin-embedded normal human colon*, performed on a Leica BOND. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab197920, 1/100 dilution, for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
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![Western blot - HRP Anti-COX IV antibody [20E8C12] (ab197920)](https://www.abcam.com/ps/products/197/ab197920/Images/ab197920-238356-anti-cox-iv-antibody-20e8c12-hrp-western-blot.jpg)
Western blot - HRP Anti-COX IV antibody [20E8C12] (ab197920)HRP Anti-COX IV antibody [20E8C12] (ab197920) at 1/5000 dilution + Human heart tissue lysate - mitochondrial extract (ab110337) at 5 µg
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 20 kDa
Observed band size: 16 kDa why is the actual band size different from the predicted?
Exposure time: 10 secondsThis blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab197920 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
