Anti-CD44 antibody [1M7.8.1] (ab119348)
![Anti-CD44 antibody [1M7.8.1] (ab119348)](https://www.abcam.com/ps/products/119/ab119348/Images/ab119348-444785-ab272229444774AntiCD44antibody1M781immunocytochemistrynih3t3mouse.jpg "Anti-CD44 antibody [1M7.8.1] (ab119348)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rat monoclonal [1M7.8.1] to CD44
- Suitable for: ICC
- Reacts with: Mouse, Human
Overview
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Product name
Anti-CD44 antibody [1M7.8.1]
See all CD44 primary antibodies -
Description
Rat monoclonal [1M7.8.1] to CD44 -
Host species
Rat -
Specificity
Detects a standard 85-kDa isoform of CD44 and a number of high molecular mass variant isoforms. -
Tested Applications & Species
See all applications and species dataApplication Species ICC MouseHuman
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Immunogen
Full length protein corresponding to Mouse CD44.
Database link: P15379 -
Positive control
- ICC: HeLa and NIH/3T3 cells.
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General notes
This product has switched from a hybridoma to recombinant production method on 02nd November 2020
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
1M7.8.1 -
Isotype
IgG2b -
Research areas
Images
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Immunocytochemistry - Anti-CD44 antibody [1M7.8.1] (ab119348)
NIH/3T3 (Mouse embryo fibroblast cell line) cells were fixed in 4% PFA and permeabilized with 0.1% Triton X-100. Primary antibody, ab119348 at 1/250 was incubated overnight at 4° C, followed by AlexaFluor® 488-conjugated Goat anti-Rat secondary antibody (ab150157) at 1/1000 dilution at RT for 45 min. ab179513 Anti-beta Tubulin, used as a counterstain at 1/200 dilution, was co-incubated with ab119348 overnight at 4° C, followed by Alexa Fluor® 594 Goat Anti-Rabbit secondary (ab150080) at 1/1000 dilution at RT for 45 min. Nucleus were visualized using DAPI.
Confocal image showing strong membranous staining in NIH/3T3 cells. -
![Immunocytochemistry - Anti-CD44 antibody [1M7.8.1] (ab119348)](https://www.abcam.com/ps/products/119/ab119348/Images/ab119348-444784-antiCD44-antibodyICC.jpg)
Immunocytochemistry - Anti-CD44 antibody [1M7.8.1] (ab119348)HeLa (Human epithelial cell line from cervix adenocarcinoma) cells were fixed in 4% PFA and permeabilized with 0.1% Triton X-100. Primary antibody, ab119348 at 1/250 was incubated overnight at 4° C, followed by AlexaFluor® 488-conjugated Goat anti-Rat secondary antibody (ab150157) at 1/1000 dilution at RT for 45 min. ab179513 Anti-beta Tubulin, used as a counterstain at 1/200 dilution, was co-incubated with ab119348 overnight at 4° C, followed by Alexa Fluor® 594 Goat Anti-Rabbit secondary (ab150080) at 1/1000 dilution at RT for 45 min. Nucleus were visualized using DAPI.
Confocal image showing strong membranous staining in HeLa cells.
Low expression control: MCF7 (PMID: 23039365).
