Anti-Optineurin antibody (ab264242)
Key features and details
- Rabbit polyclonal to Optineurin
- Suitable for: IP, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Optineurin antibody
See all Optineurin primary antibodies -
Description
Rabbit polyclonal to Optineurin -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
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Immunogen
Synthetic peptide within Human Optineurin aa 504-554. The exact sequence is proprietary. NP_068815.2
Database link: Q96CV9 -
Positive control
- WB: HeLa and HEK-293T whole cell lysates. IP: HeLa whole cell lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Optineurin antibody (ab264242)All lanes : Anti-Optineurin antibody (ab264242) at 0.4 µg/ml
Lane 1 : HeLa human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 66 kDa
Exposure time: 30 secondsLysates prepared using NETN buffer.
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Immunoprecipitation - Anti-Optineurin antibody (ab264242)Optineurin was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) using ab264242 at 6 μg per reaction. Western blot was performed on the immunoprecipitates using ab264242 at 1 μg/ml.
Lane 1: ab264242 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in Hela whole cell lysate.
Exposure time: 30 seconds.
Lysates prepared in NETN buffer.
