Anti-HLA B7 antibody [BB7.1] (ab20169)
![Anti-HLA B7 antibody [BB7.1] (ab20169)](https://www.abcam.com/ps/products/20/ab20169/Images/ab20169-29514-anti-hla-b7-antibody-bb71-flow-cytometry.jpg "Anti-HLA B7 antibody [BB7.1] (ab20169)")
Key features and details
- Mouse monoclonal [BB7.1] to HLA B7
- Suitable for: Flow Cyt
- Reacts with: Human, Monkey
- Isotype: IgG1
Overview
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Product name
Anti-HLA B7 antibody [BB7.1]
See all HLA B7 primary antibodies -
Description
Mouse monoclonal [BB7.1] to HLA B7 -
Host species
Mouse -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Human, Monkey -
Immunogen
Full length protein (Human). Papain solubilised HLA-A2, B7
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General notes
Human Leukocyte Antigens are highly polymorphic proteins that are involved in the presentation of antigens to the T-cell receptor. There are two classes of HLA antigens, class I (HLA-A, HLA-B and HLA-C) and class II (HLA-D). This antibody can be used for HLA typing.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Constituent: PBS -
Concentration information loading... -
Purity
Protein G purified -
Primary antibody notes
Human Leukocyte Antigens are highly polymorphic proteins that are involved in the presentation of antigens to the T-cell receptor. There are two classes of HLA antigens, class I (HLA-A, HLA-B and HLA-C) and class II (HLA-D). This antibody can be used for HLA typing. -
Clonality
Monoclonal -
Clone number
BB7.1 -
Myeloma
NS1 -
Isotype
IgG1 -
Light chain type
unknown -
Research areas
Images
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Flow Cytometry - Anti-HLA B7 antibody [BB7.1] (ab20169)Overlay histogram showing Jurkat cells stained with ab20169 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (a20169, 2µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
