Anti-PKC epsilon antibody (ab233292)
Key features and details
- Rabbit polyclonal to PKC epsilon
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Human, Pig
- Isotype: IgG
Overview
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Product name
Anti-PKC epsilon antibody
See all PKC epsilon primary antibodies -
Description
Rabbit polyclonal to PKC epsilon -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Human, Pig
Predicted to work with: Rat, Rabbit
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Immunogen
Recombinant fragment (His-tag) corresponding to Human PKC epsilon aa 400-700. (Expressed in E.coli).
Database link: Q02156 -
Positive control
- WB: Recombinant human PKC epsilon; Mouse and pig brain lysates; HeLa cell lysate. IHC-P: Human liver, kidney, rectum and thyroid cancer tissues.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab233292 was purified by antigen-specific affinity chromatography followed by Protein A affinity chromatography. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC epsilon antibody (ab233292)
Paraffin-embedded human liver tissue stained for PKC epsilon using ab233292 at 10 μg/ml in immunohistochemical analysis. DAB staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC epsilon antibody (ab233292)Paraffin-embedded human kidney tissue stained for PKC epsilon using ab233292 at 20 μg/ml in immunohistochemical analysis. DAB staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC epsilon antibody (ab233292)Paraffin-embedded human rectum tissue stained for PKC epsilon using ab233292 at 20 μg/ml in immunohistochemical analysis. DAB staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC epsilon antibody (ab233292)Paraffin-embedded human thyroid cancer tissue stained for PKC epsilon using ab233292 at 20μg/ml in immunohistochemical analysis. DAB staining.
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Western blot - Anti-PKC epsilon antibody (ab233292)Anti-PKC epsilon antibody (ab233292) at 3 µg/ml + Recombinant human PKC epsilon protein
Secondary
HRP-Linked Guinea pig Anti-Rabbit at 1/2000 dilution
Predicted band size: 84 kDa
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Western blot - Anti-PKC epsilon antibody (ab233292)All lanes : Anti-PKC epsilon antibody (ab233292) at 3 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 2 : Mouse brain lysate
Lane 3 : Pig brain lysate
Secondary
All lanes : HRP-Linked Guinea pig Anti-Rabbit at 1/2000 dilution
Predicted band size: 84 kDa