Antibodies, Proteins, Kits and Reagents for Life Science

348 441 ₸ Product size

100 µl 348 441 ₸

Order now and get it on Thursday February 25, 2021

PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab209478)

Product name

PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control
See all IgG isotype controls
Conjugation

PE. Ex: 488nm, Em: 575nm
Tested applications

Suitable for: ICC/IF, Flow Cytmore details
Immunogen

Chemical/ Small Molecule conjugated to keyhole limpet haemocyanin. KLH is a copper containing oxygen carrier occurring freely dissolved in the hemolymph of many molluscs and arthropods.KLH forms a large complex composed of ~50 kDa subunits.
General notes
KLH is often used in molecular immunology as a carrier protein conjugated to low molecular weight molecules such as peptides, amino acids, nucleic acids, drugs or toxins to render them more immunogenic due to the size of the conjugate complex and the immunogenicity of KLH.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Upon delivery aliquot. Store at +4°C. Avoid freeze / thaw cycle. Store In the Dark.
Storage buffer

pH: 7.40
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Immunogen affinity purified
Clonality

Monoclonal
Clone number

EPR25A
Isotype

IgG
Research areas
Cellular localization

Secreted
Alternative names
- Ig gamma 1 chain C region
- Ig gamma 2 chain C region
- Ig gamma 3 chain C region
- Ig gamma 4 chain C region
- IgG
- IGHG1
- IGHG2
- IGHG3
- IGHG4
- Immunoglobin heavy constant gamma 1
- Immunoglobulin G
see all

Immunocytochemistry - PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab209478)

Immunofluorescent analysis of HeLa (human cervical cancer) cells, fixed with 4% formaldehyde (10 min). The cells were permeabilized with 0.1% Triton X-100 for 5 minutes, blocked with Image-iT^® FX Signal Enhancer at room temperature for 30 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab209478 (Rabbit IgG, monoclonal [EPR25A] - Isotype Control) at 1/50 dilution (showing no signal) and ab195884, Rat monoclonal [YOL1/34] to Tubulin Microtubule Marker (Alexa Fluor® 647), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

This product also gave a positive signal under the same testing conditions in HeLa cells fixed with 100% methanol (5min).
Flow Cytometry - PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab209478)

Flow cytometry overlay histogram showing HeLa cells stained with ab209446 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 90% Methanol for 30 min at -20°C. The cells were incubated in 1x PBS containing 10% normal goat serum to block non-specific protein-protein interaction followed by the antibody (ab209446) (1x10⁶ in 100µL at 0.01µg/mL, 0.1µg/mL, 1µg/mL (1/50000, 1/5000, 1/500)) for 30 min at 22°C.

Isotype control antibody (black line) was used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

Acquisition of >5000 events were collected using a 50 mW Yellow/Green laser (561nm) and 586/15 bandpass filter.
Flow Cytometry - PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab209478)

Overlay histogram showing HeLa cells stained with ab208741 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab208741, 1/500 dilution) for 30 min at 22ºC.

Isotype control antibody (black line) was rabbit IgG monoclonal [EPR25A] (ab209478) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

Acquisition of >5,000 events were collected using a 20 mW Solid State Blue Laser (488nm) and 585/40 bandpass filter.
PE Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab209478)

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