Antibodies, Proteins, Kits and Reagents for Life Science

298 185 ₸ Product size

100 µl 298 185 ₸

Order now and get it on Tuesday March 02, 2021

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [RIGG-69] to IgG
Suitable for: WB, ELISA
Reacts with: Mouse, Rat, Human

Product name

Anti-IgG antibody [RIGG-69]
See all IgG primary antibodies
Description

Rabbit monoclonal [RIGG-69] to IgG
Host species

Rabbit
Tested applications

Suitable for: WB, ELISAmore details
Species reactivity

Reacts with: Mouse, Rat, Human
Immunogen

Full length protein corresponding to Rat IgG.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 99% PBS
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

RIGG-69
Isotype

IgG
Research areas

Western blot - Anti-IgG antibody [RIGG-69] (ab133470)

All lanes : purified at 1/1000 dilution

Lane 1 : Human tonsil tissue lysate
Lane 2 : Human heart tissue lysate
Lane 3 : Mouse heart tissue lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : HRP anti-rabbit IgG, specific to the non-reduced form of IgG at 1/1000 dilution

Observed band size: 50 kDa
why is the actual band size different from the predicted?

Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
ELISA - Anti-IgG antibody [RIGG-69] (ab133470)

Direct ELISA antibody dose-response curve using ab133470 between 0-1000 ng/ml. Antigen concentration of 1000 ng/mL. An alkaline phosphatase-conjugated goat anti-rabbit IgG (H+L) (1/2500) was used as the secondary antibody.
Western blot - Anti-IgG antibody [RIGG-69] (ab133470)

All lanes : Anti-IgG antibody [RIGG-69] (ab133470) at 1/150 dilution (unpurified)

Lane 1 : Human tonsil tissue lysate
Lane 2 : Human heart tissue lysate
Lane 3 : Mouse heart tissue lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : HRP anti-rabbit IgG, specific to the non-reduced form of IgG at 1/1000 dilution

Observed band size: 50 kDa why is the actual band size different from the predicted?

Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
ELISA - Anti-IgG antibody [RIGG-69] (ab133470)

Antibody dose-response curve using purified ab133470. Antigen concentration of 1 µg/mL. An Alkaline Phosphatase-conjugated goat anti-rabbit IgG (H+L) (1/2500) was used as the secondary antibody.
Western blot - Anti-IgG antibody [RIGG-69] (ab133470)

All lanes : Anti-IgG antibody [RIGG-69] (ab133470) (unpurified)

Lane 1 : Rat spleen
Lane 2 : Human tonsil
ELISA - Anti-IgG antibody [RIGG-69] (ab133470)

Antibody dose-response curve using unpurified ab133470. Antigen concentration of 1 µg/mL. An Alkaline Phosphatase-conjugated goat anti-rabbit IgG (H+L) (1/2500) was used as the secondary antibody.
Anti-IgG antibody [RIGG-69] (ab133470)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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