Antibodies, Proteins, Kits and Reagents for Life Science

298 185 ₸ Product size

100 µl 298 185 ₸ 10 µl 123 964 ₸

Order now and get it on Tuesday March 02, 2021

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR6321] to CBFb
Suitable for: WB
Knockout validated
Reacts with: Human

Product name

Anti-CBFb antibody [EPR6321]
See all CBFb primary antibodies
Description

Rabbit monoclonal [EPR6321] to CBFb
Host species

Rabbit
Tested Applications & Species

Application Species
WB
Human

See all applications and species data
Immunogen

Synthetic peptide within Human CBFb aa 1-100. The exact sequence is proprietary.
Positive control
- WB: A431, HeLa, K562, Jurkat and HUVEC cell lysates.
General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Dissociation constant (K_D)

K_D = 1.97 x 10 ^-10 M
Learn more about K_D
Storage buffer

pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR6321
Isotype

IgG
Research areas

Western blot - Anti-CBFb antibody [EPR6321] (ab124693)

All lanes : Anti-CBFb antibody [EPR6321] (ab124693) at 1/1000 dilution

Lane 1 : Wild-type A431 cell lysate
Lane 2 : CBFB knockout A431 cell lysate
Lane 3 : K562 cell lysate
Lane 4 : HeLa cell lysate

Lysates/proteins at 20 µg per lane.

Performed under reducing conditions.

Predicted band size: 22 kDa
Observed band size: 24-25 kDa
why is the actual band size different from the predicted?

Lanes 1 - 4: Merged signal (red and green). Green - ab124693 observed at 24-25 kDa. Red - loading control, ab7291 (Mouse anti-Alpha Tubulin [DM1A] observed at 55kDa.

ab124693 was shown to react with CBFb in wild-type A431 cells in western blot. Loss of signal was observed when CBFB knockout sample was used. Wild-type and CBFB knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween^®) before incubation with ab124693 and ab7291 (Mouse anti-Alpha Tubulin [DM1A] overnight at 4°C at a 1 in 1000 Dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
Western blot - Anti-CBFb antibody [EPR6321] (ab124693)

All lanes : Anti-CBFb antibody [EPR6321] (ab124693) at 1/1000 dilution

Lane 1 : K562 cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : HUVEC cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat anti-Rabbit HRP at 1/2000 dilution

Predicted band size: 22 kDa
OI-RD Scanning - Anti-CBFb antibody [EPR6321] (ab124693)

Equilibrium disassociation constant (K_D)
Learn more about K_D

Click here to learn more about K_D
Anti-CBFb antibody [EPR6321] (ab124693)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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