Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CBFb antibody [EPR6321] (ab124693)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR6321] to CBFb
  • Suitable for: WB
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-CBFb antibody [EPR6321]
    See all CBFb primary antibodies

  • Description

    Rabbit monoclonal [EPR6321] to CBFb

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human CBFb aa 1-100. The exact sequence is proprietary.

  • Positive control

    • WB: A431, HeLa, K562, Jurkat and HUVEC cell lysates.

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.

  • Dissociation constant (KD)

    KD = 1.97 x 10 -10 M
    Learn more about KD

  • Storage buffer

    pH: 7.20
    Preservative: 0.05% Sodium azide
    Constituents: 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR6321

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-CBFb antibody [EPR6321] (ab124693)
    Western blot - Anti-CBFb antibody [EPR6321] (ab124693)
    All lanes : Anti-CBFb antibody [EPR6321] (ab124693) at 1/1000 dilution

    Lane 1 : Wild-type A431 cell lysate
    Lane 2 : CBFB knockout A431 cell lysate
    Lane 3 : K562 cell lysate
    Lane 4 : HeLa cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 22 kDa
    Observed band size: 24-25 kDa
    why is the actual band size different from the predicted?



    Lanes 1 - 4: Merged signal (red and green). Green - ab124693 observed at 24-25 kDa. Red - loading control, ab7291 (Mouse anti-Alpha Tubulin [DM1A] observed at 55kDa.

    ab124693 was shown to react with CBFb in wild-type A431 cells in western blot. Loss of signal was observed when CBFB knockout sample was used. Wild-type and CBFB knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween®) before incubation with ab124693 and ab7291 (Mouse anti-Alpha Tubulin [DM1A] overnight at 4°C at a 1 in 1000 Dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-CBFb antibody [EPR6321] (ab124693)
    Western blot - Anti-CBFb antibody [EPR6321] (ab124693)
    All lanes : Anti-CBFb antibody [EPR6321] (ab124693) at 1/1000 dilution

    Lane 1 : K562 cell lysate
    Lane 2 : Jurkat cell lysate
    Lane 3 : HUVEC cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit HRP at 1/2000 dilution

    Predicted band size: 22 kDa

  • OI-RD Scanning - Anti-CBFb antibody [EPR6321] (ab124693)
    OI-RD Scanning - Anti-CBFb antibody [EPR6321] (ab124693)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-CBFb antibody [EPR6321] (ab124693)
    Anti-CBFb antibody [EPR6321] (ab124693)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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