Anti-CBFb antibody - ChIP Grade (ab195411)
Key features and details
- Rabbit polyclonal to CBFb - ChIP Grade
- Suitable for: ChIP-sequencing, ChIP
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-CBFb antibody - ChIP Grade
See all CBFb primary antibodies -
Description
Rabbit polyclonal to CBFb - ChIP Grade -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ChIP HumanChIP-seq Human
-
Immunogen
This product was produced with the following immunogens:
Synthetic peptide corresponding to Human CBFb (internal sequence) conjugated to keyhole limpet haemocyanin.
Database link: Q13951
Synthetic peptide corresponding to Human CBFb (internal sequence) conjugated to keyhole limpet haemocyanin.
Database link: Q13951 -
Positive control
- Chromatin from SKNO-1 cells.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide -
Concentration information loading... -
Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
-
ChIP - Anti-CBFb antibody - ChIP Grade (ab195411)
ChIP results obtained with ab195411 directed against CBFb
ChIP assays were performed using SKNO-1 cells, ab195411 and optimized primer pairs for qPCR. Sheared chromatin from 1.25 million cells and 4 µl of antibody were used per ChIP experiment. QPCR was performed using primers specific for the FUT7, OGG1, NFE2, and SPI1 genes. Figure shows the relative occupancy, calculated as the ratio + control/background for which the MYOG gene was used.
-
ChIP-sequencing - Anti-CBFb antibody - ChIP Grade (ab195411)ChIP assays were performed using SKNO-1 cells, ab195411 and optimized primer pairs for qPCR. Sheared chromatin from 1.25 million cells and 4 μl of antibody were used per ChIP experiment. The IP’d DNA from 6 ChIP’s was pooled and analysed with an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 32 bp tags were aligned to the human reference genome (hg18) using the ELAND algorithm. This figure shows the results of the complete chromosome 3 and three genomic regions region surrounding the OGG1, FUT7 and NFE2 genes, respectively. The position of the PCR amplicon is indicated with an arrow.
