Anti-CBFb antibody (ab125191)
Key features and details
- Rabbit polyclonal to CBFb
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-CBFb antibody
See all CBFb primary antibodies -
Description
Rabbit polyclonal to CBFb -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB MouseHuman
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Immunogen
Synthetic peptide, corresponding to a region within amino acids 166-187 of Human CBFb (NP_074036.1).
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Positive control
- WB: HeLa, HEK-293T, TCMK, NIH/3T3 and Jurkat whole cell lysate. IP: Jurkat whole cell lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-CBFb antibody (ab125191)All lanes : Anti-CBFb antibody (ab125191) at 0.1 µg/ml
Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 4 : TCMK (Mouse kidney epithelial cell line) whole cell lysate
Lane 5 : NIH/3T3 (Mouse embryo fibroblast cell line) whole cell lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 22 kDa
Exposure time: 30 secondsCells prepared using NETN lysis buffer.
4-12% SDS-PAGE.
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Immunoprecipitation - Anti-CBFb antibody (ab125191)Detection of human CBFb by western blot of immunoprecipitates.
Lane 1: Sample.
Lane 2: Control IgG.
Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Jurkat (Human T cell leukemia cell line from peripheral blood) cells prepared using NETN lysis buffer.
Antibodies: ab125191 used for IP at 3 μg per reaction.For blotting immunoprecipitated CBFb ab125191 was used at 1 μg/ml.
Detection: Chemiluminescence with an exposure time of 30 seconds.
