Anti-AP2 alpha + beta antibody [A6/2/2] (ab61)
![Anti-AP2 alpha + beta antibody [A6/2/2] (ab61)](https://www.abcam.com/ps/products/0/ab61/Images/ab61-3272-anti-ap2-alpha-beta-antibody-a6-2-2-immunofluorescence.jpg "Anti-AP2 alpha + beta antibody [A6/2/2] (ab61)")
Key features and details
- Mouse monoclonal [A6/2/2] to AP2 alpha + beta
- Suitable for: ICC/IF
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-AP2 alpha + beta antibody [A6/2/2] -
Description
Mouse monoclonal [A6/2/2] to AP2 alpha + beta -
Host species
Mouse -
Specificity
Reacts with both AP-2 alpha and beta forms. As the antibody also recognises AP2 beta, the epitope it recognises is probably the last 8 amino acids as these are common between alpha and beta. -
Tested applications
Suitable for: ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
C-terminal peptide of AP-2 alpha (PNSHTDNNAKSSDKEEKHRK).
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Positive control
- Raji cells or breast carcinoma
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
A6/2/2 -
Myeloma
NS0 -
Isotype
IgG1 -
Light chain type
unknown -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-AP2 alpha + beta antibody [A6/2/2] (ab61)ICC/IF image of ab61 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab61, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 Goat anti-Mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.