Human Arginase 1 ELISA Kit (ab230930)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 17.77 pg/ml
- Range: 187.5 pg/ml - 12000 pg/ml
- Sample type: Tissue Extracts
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human Arginase 1 ELISA Kit
See all Liver Arginase kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Tissue 8 2.2% Inter-assay Sample n Mean SD CV% Tissue 3 4.4% -
Sample type
Tissue Extracts -
Assay type
Sandwich (quantitative) -
Sensitivity
17.77 pg/ml -
Range
187.5 pg/ml - 12000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Tissue Extracts 103 99% - 107% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human Arginase 1 ELISA Kit (ab230930) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Arginase 1 protein in tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Arginase 1 with 17.77 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human Arginase 1 Capture Antibody 1 x 600µl 10X Human Arginase 1 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent 4BI 1 x 6ml Human Arginase 1 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 12ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Pathway
Nitrogen metabolism; urea cycle; L-ornithine and urea from L-arginine: step 1/1. -
Involvement in disease
Defects in ARG1 are the cause of argininemia (ARGIN) [MIM:207800]; also known as hyperargininemia. Argininemia is a rare autosomal recessive disorder of the urea cycle. Arginine is elevated in the blood and cerebrospinal fluid, and periodic hyperammonemia occurs. Clinical manifestations include developmental delay, seizures, mental retardation, hypotonia, ataxia, progressive spastic quadriplegia. -
Sequence similarities
Belongs to the arginase family. -
Cellular localization
Cytoplasm. - Information by UniProt
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Alternative names
- A I
- Al
- ARG 1
see all -
Database links
- Entrez Gene: 383 Human
- Omim: 608313 Human
- SwissProt: P05089 Human
- Unigene: 440934 Human
Images
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Other - Human Arginase 1 ELISA Kit (ab230930)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of human Arginase 1 standard curve in 1X Cell Extraction Buffer PTR.Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native Arginase 1 in human liver extract based on a 5 µg/mL extract load.The concentrations of Arginase 1 were measured in duplicate and interpolated from the Arginase 1 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Arginase 1 concentration was determined to be 6383.5 pg/mL in 5 µg /mL liver extract.
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Sandwich ELISA - Human Arginase 1 ELISA Kit (ab230930)To learn more about the advantages of recombinant antibodies see here.
