Human Lysozyme ELISA Kit (LZM) (ab108880)
Key features and details
- Sensitivity: 0.15 ng/ml
- Range: 0.156 ng/ml - 5 ng/ml
- Sample type: Cell culture supernatant, Cerebral Spinal Fluid, Milk, Other biological fluids, Plasma, Saliva, Serum, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human Lysozyme ELISA Kit (LZM)
See all Lysozyme kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall 5% Inter-assay Sample n Mean SD CV% Overall 7.1% -
Sample type
Cell culture supernatant, Saliva, Milk, Urine, Serum, Plasma, Other biological fluids, Cerebral Spinal Fluid -
Assay type
Sandwich (quantitative) -
Sensitivity
= 0.15 ng/ml -
Range
0.156 ng/ml - 5 ng/ml -
Recovery
= 97.5 %
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Assay time
4h 00m -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Human Lysozyme (LZM) in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of human Lysozyme in plasma, serum, urine, saliva, CSF and cell culture supernatants.
A Lysozyme specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Lysozyme specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Lysozyme captured in plate.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
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Platform
Microplate
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 100X Streptavidin-Peroxidase Conjugate 1 x 80µl 10X Diluent N Concentrate 1 x 30ml 20X Wash Buffer Concentrate 2 x 30ml 50X Biotinylated Human Lysozyme Antibody 1 x 120µl Chromogen Substrate 1 x 8ml Lysozyme Microplate (12 x 8 well strips) 1 unit Lysozyme Standard 1 vial Sealing Tapes 3 units Stop Solution 1 x 12ml -
Research areas
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Function
Lysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte-macrophage system and enhance the activity of immunoagents. -
Involvement in disease
Amyloidosis 8 -
Sequence similarities
Belongs to the glycosyl hydrolase 22 family. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- 1
- 1 4 beta N acetylmuramidase C
- 4-beta-N-acetylmuramidase C
see all -
Database links
- Entrez Gene: 4069 Human
- Omim: 153450 Human
- SwissProt: P61626 Human
- Unigene: 524579 Human
Images
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ELISA data
Human lysozyme measured in biological fluids showing quantity (ng) per mL of tested sample. Human serum and plasma diluted 1000-9000 fold. Human saliva diluted 8000-24000 fold. Human urine diluted 10 fold.
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Typical Standard CurveRepresentative Standard Curve using ab108880.
