Human Lysozyme ELISA Kit (LZM) (ab108877)
Key features and details
- Sensitivity: 0.09 µg/ml
- Range: 0.093 µg/ml - 6 µg/ml
- Sample type: Milk
- Detection method: Colorimetric
- Assay type: Competitive
- Reacts with: Human
Overview
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Product name
Human Lysozyme ELISA Kit (LZM)
See all Lysozyme kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall 4.5% Inter-assay Sample n Mean SD CV% Overall 8.7% -
Sample type
Milk -
Assay type
Competitive -
Sensitivity
= 0.09 µg/ml -
Range
0.093 µg/ml - 6 µg/ml -
Recovery
= 98 %
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Assay time
3h 00m -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Abcam’s Lysozyme (LZM) Human in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Human Lysozyme in milk.
A Lysozyme specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently biotinylated Lysozyme is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is inversely proportional to the amount of Lysozyme captured in plate.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
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Platform
Microplate
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 100X Streptavidin-Peroxidase Conjugate 1 x 80µl 10X Diluent M Concentrate 1 x 30ml 20X Wash Buffer Concentrate 1 x 30ml Biotinylated Human Lysozyme (Lyophilized) 1 vial Chromogen Substrate 1 x 7ml Lysozyme Microplate (12 x 8 well strips) 1 unit Lysozyme Standard 1 vial Sealing Tapes 3 units Stop Solution 1 x 11ml -
Research areas
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Function
Lysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte-macrophage system and enhance the activity of immunoagents. -
Involvement in disease
Amyloidosis 8 -
Sequence similarities
Belongs to the glycosyl hydrolase 22 family. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- 1
- 1 4 beta N acetylmuramidase C
- 4-beta-N-acetylmuramidase C
see all -
Database links
- Entrez Gene: 4069 Human
- Omim: 153450 Human
- SwissProt: P61626 Human
- Unigene: 524579 Human
Images
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Typical Standard Curve
Representative Standard Curve using ab108877.
