Anti-IL-10RB/IL-10R2 antibody [EPR24691-11] (ab273038)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR24691-11] to IL-10RB/IL-10R2
- Suitable for: Flow Cyt
- Reacts with: Human
Overview
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Product name
Anti-IL-10RB/IL-10R2 antibody [EPR24691-11]
See all IL-10RB/IL-10R2 primary antibodies -
Description
Rabbit monoclonal [EPR24691-11] to IL-10RB/IL-10R2 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanWB Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Flow Cyt: Human PBMC cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR24691-11 -
Isotype
IgG -
Research areas
Images
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Flow cytometric analysis of Human peripheral blood mononuclear cell (PBMC) cells labelling IL-10RB/IL-10R2 with ab273038 at 1/500 dilution (0.1ug) (Right) compared with a Rabbit monoclonal IgG (ab172730) isotype control (Left). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody. Cells were stained with rabbit IgG or ab273038. Then stained with anti-CD14 conjugated to BV510. Gated on viable cells.
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Flow cytometric analysis of Human peripheral blood mononuclear cell (PBMC) cells labelling IL-10RB/IL-10R2 with ab273038 at 1/500 dilution (0.1ug) (Right) compared with a Rabbit monoclonal IgG (ab172730) isotype control (Left) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody. Cells were stained with rabbit IgG or ab273038. Then stained with anti-CD3 conjugated to Alexa Fluor® 647. Gated on viable cells.
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Flow cytometric analysis of Human peripheral blood mononuclear cell (PBMC) cells labelling IL-10RB/IL-10R2 with ab273038 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody. Cells were stained with rabbit IgG or ab273038. Then stained with anti-CD3 conjugated to Alexa Fluor® 647 and anti-CD14 conjugated to BV510. Gated on viable T cells and monocytes.
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Flow cytometric analysis of Human peripheral blood mononuclear cell (PBMC) cells labelling IL-10RB/IL-10R2 with ab273038 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody. Cells were stained with rabbit IgG or ab273038. Then stained with anti-CD56 conjugated to PE. Gated on viable NK cells.
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