Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-THBS4 antibody [EPR22922-232] - BSA and Azide free (ab264564)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22922-232] to THBS4 - BSA and Azide free
  • Suitable for: WB, ICC/IF, IP, Flow Cyt (Intra)
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-THBS4 antibody [EPR22922-232] - BSA and Azide free
    See all THBS4 primary antibodies

  • Description

    Rabbit monoclonal [EPR22922-232] to THBS4 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, ICC/IF, IP, Flow Cyt (Intra)more details
    Unsuitable for: IHC-P

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human skeletal muscle, human serum, mouse skeletal muscle, human articular cartilage, mouse kidney, mouse articular cartilage, rat heart and rat articular cartilage lysates. ICC/IF: C2C12 and L6 cells. Flow Cyt (intra): C2C12 and L6 cells. IP: Human and mouse skeletal muscle whole cell lysate.

  • General notes

    ab264564 is the carrier-free version of ab263898.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunoprecipitation - Anti-THBS4 antibody [EPR22922-232] - BSA and Azide free (ab264564)
    Immunoprecipitation - Anti-THBS4 antibody [EPR22922-232] - BSA and Azide free (ab264564)

    THBS4 was immunoprecipitated from 0.35 mg mouse skeletal muscle lysate 10µg with ab263898 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab263898 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/1000 dilution.

    Lane 1: Mouse skeletal muscle lysate 10µg

    Lane 2: ab263898 IP in mouse skeletal muscle lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab263868 in mouse skeletal muscle lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 seconds.

    The expression profile and molecular weight observed is consistent with what has been described in the literature (PMID:30669608).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab263898).

  • Immunoprecipitation - Anti-THBS4 antibody [EPR22922-232] - BSA and Azide free (ab264564)
    Immunoprecipitation - Anti-THBS4 antibody [EPR22922-232] - BSA and Azide free (ab264564)

    THBS4 was immunoprecipitated from 0.35 mg human skeletal muscle lysate 10µg with ab263898 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab263898 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/1000 dilution.

    Lane 1: Human skeletal muscle lysate 10µg

    Lane 2: ab263898 IP in human skeletal muscle lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab263868 in human skeletal muscle lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 seconds.

    The expression profile and molecular weight observed is consistent with what has been described in the literature (PMID:30669608).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab263898).

  • Immunocytochemistry/ Immunofluorescence - Anti-THBS4 antibody [EPR22922-232] - BSA and Azide free (ab264564)
    Immunocytochemistry/ Immunofluorescence - Anti-THBS4 antibody [EPR22922-232] - BSA and Azide free (ab264564)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized L6 (Rat skeletal muscle myoblast) cells labeling THBS4 with ab263898 at 1/100 (6.9 µg/ml) dilution, followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 (2 µg/ml) dilution (Green). Confocal image showing cytoplasmic staining in L6 cell line is observed. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 (2 µg/ml) dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab263898).

  • Immunocytochemistry/ Immunofluorescence - Anti-THBS4 antibody [EPR22922-232] - BSA and Azide free (ab264564)
    Immunocytochemistry/ Immunofluorescence - Anti-THBS4 antibody [EPR22922-232] - BSA and Azide free (ab264564)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized C2C12 (Mouse myoblast) cells labeling THBS4 with ab263898 at 1/100 (6.9 µg/ml) dilution, followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 (2 µg/ml) dilution (Green). Confocal image showing cytoplasmic staining in C2C12 cell line is observed. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 (2 µg/ml) dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab263898).

  • Flow Cytometry - Anti-THBS4 antibody [EPR22922-232] - BSA and Azide free (ab264564)
    Flow Cytometry - Anti-THBS4 antibody [EPR22922-232] - BSA and Azide free (ab264564)

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized L6 (Rat skeletal muscle myoblast) cells labeling THBS4 with ab263898 at 1/700 (Red) compared with a Rabbit monoclonal IgG (ab172730) / Black isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab263898).

  • Flow Cytometry - Anti-THBS4 antibody [EPR22922-232] - BSA and Azide free (ab264564)
    Flow Cytometry - Anti-THBS4 antibody [EPR22922-232] - BSA and Azide free (ab264564)

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized C2C12 (Mouse myoblasts myoblast) cells labeling THBS4 with ab263898 at 1/700 (Red) compared with a Rabbit monoclonal IgG (ab172730) / Black isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab263898).

  • Anti-THBS4 antibody [EPR22922-232] - BSA and Azide free (ab264564)
    Anti-THBS4 antibody [EPR22922-232] - BSA and Azide free (ab264564)

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