Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CrkL antibody [Y244] - BSA and Azide free (ab247210)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [Y244] to CrkL - BSA and Azide free
  • Suitable for: ICC, WB, IHC-P, IP
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-CrkL antibody [Y244] - BSA and Azide free
    See all CrkL primary antibodies

  • Description

    Rabbit monoclonal [Y244] to CrkL - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    This antibody recognises CrkL (Crk-like)protein.

  • Tested applications

    Suitable for: ICC, WB, IHC-P, IPmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa and K562 cell lysates. ICC: K-562 cells IP: K-562 whole cell lysate.

  • General notes

    ab247210 is the carrier-free version of ab32018 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab247210 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-CrkL antibody [Y244] - BSA and Azide free (ab247210)
    Western blot - Anti-CrkL antibody [Y244] - BSA and Azide free (ab247210)
    All lanes : Anti-CrkL antibody [Y244] (ab32018) at 1/1000 dilution

    Lane 1 : Wild-type HeLa cell lysate
    Lane 2 : CRKL knockout HeLa cell lysate
    Lane 3 : K-562 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

    Predicted band size: 33 kDa
    Observed band size: 37 kDa
    why is the actual band size different from the predicted?



    This data was developed using the same antibody clone in a different buffer formulation (ab32018).

    Lanes 1-3: Merged signal (red and green). Green - ab32018 observed at 37 kDa. Red - loading control ab7291 observed at 50 kDa.

    ab32018 Anti-CrkL antibody [Y244] was shown to specifically react with CrkL in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265993 (knockout cell lysate ab257397) was used. Wild-type and CrkL knockout samples were subjected to SDS-PAGE. ab32018 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

     

     

  • Immunocytochemistry - Anti-CrkL antibody [Y244] - BSA and Azide free (ab247210)
    Immunocytochemistry - Anti-CrkL antibody [Y244] - BSA and Azide free (ab247210)
    This data was developed using the same antibody clone in a different buffer formulation (ab32018).
    Immunocytochemistry analysis of K-562 (human chronic myelogenous leukemia lymphoblast) labeling CrkL with purified ab32018 at 1/100 dilution (10 µg/ml). Cells were fixed with 100% methanol. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/1000 (2 µg/ml) was used as the secondary antibody. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/ml) was used as counterstain. Nuclei were stained blue with DAPI.
    Negative control: PBS instead of the primary antibody.
  • Immunoprecipitation - Anti-CrkL antibody [Y244] - BSA and Azide free (ab247210)
    Immunoprecipitation - Anti-CrkL antibody [Y244] - BSA and Azide free (ab247210)

    This data was developed using ab32018, the same antibody clone in a different buffer formulation.
    Purified ab32018 at 1/50 dilution (2µg) immunoprecipitating CrkL in K-562 whole cell lysate.
    Lane 1 (input): K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysate 10µg
    Lane 2 (+): ab32018 + K-562 whole cell lysate.
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab32018 in K-562 whole cell lysate.
    VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
    Blocking Buffer and concentration: 5% NFDM/TBST.
    Diluting buffer and concentration: 5% NFDM/TBST.
    Observed band size: 37 kDa

  • Anti-CrkL antibody [Y244] - BSA and Azide free (ab247210)
    Anti-CrkL antibody [Y244] - BSA and Azide free (ab247210)

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