Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-GDF7 antibody [EPR16000] - BSA and Azide free (ab251010)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR16000] to GDF7 - BSA and Azide free
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-GDF7 antibody [EPR16000] - BSA and Azide free
    See all GDF7 primary antibodies

  • Description

    Rabbit monoclonal [EPR16000] to GDF7 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab251010 is the carrier-free version of ab189928.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunocytochemistry - Anti-GDF7 antibody [EPR16000] - BSA and Azide free (ab251010)
    Immunocytochemistry - Anti-GDF7 antibody [EPR16000] - BSA and Azide free (ab251010)

    This data was developed using ab189928, the same antibody clone in a different buffer formulation.

    Immunocytochemical analysis of 4% paraformaldehyde fixed U87-MG cells labeling GDF7 with ab189928 at 1/50 dilution, and Goat anti rabbit IgG(Alexa Fluor® 555) at 1/200 as a secondary antibody. Counterstained with DAPI.
  • Western blot - Anti-GDF7 antibody [EPR16000] - BSA and Azide free (ab251010)
    Western blot - Anti-GDF7 antibody [EPR16000] - BSA and Azide free (ab251010)
    Anti-Spa-1 antibody [EPR14134] (ab189929) at 1/10000 dilution + Human fetal brain tissue lysate at 20 µg

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 47 kDa
    Additional bands at: 52 kDa. We are unsure as to the identity of these extra bands.



    This data was developed using ab189928, the same antibody clone in a different buffer formulation.

  • Western blot - Anti-GDF7 antibody [EPR16000] - BSA and Azide free (ab251010)
    Western blot - Anti-GDF7 antibody [EPR16000] - BSA and Azide free (ab251010)
    All lanes : Anti-GDF7 antibody [EPR16000] (ab189928) at 1/1000 dilution

    Lane 1 : C6 cell lysate
    Lane 2 : Neuro-2a cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 47 kDa



    This data was developed using ab189928, the same antibody clone in a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GDF7 antibody [EPR16000] - BSA and Azide free (ab251010)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GDF7 antibody [EPR16000] - BSA and Azide free (ab251010)
    This data was developed using ab189928, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of formalin fixed, paraffin embedded Human adenocarcinoma of colon tissue labeling GDF7 with ab189928 at 1/2000 dilution, and prediluted HRP Polymer for Rabbit IgG as a secondary antibody. Counterstained with Hematoxylin. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GDF7 antibody [EPR16000] - BSA and Azide free (ab251010)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GDF7 antibody [EPR16000] - BSA and Azide free (ab251010)
    This data was developed using ab189928, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of formalin fixed, paraffin embedded Human adenocarcinoma of colon labeling GDF7 with ab189928 at 1/2000 dilution, and prediluted HRP Polymer for Rabbit IgG as a secondary antibody. Counterstained with Hematoxylin. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Anti-GDF7 antibody [EPR16000] - BSA and Azide free (ab251010)
    Anti-GDF7 antibody [EPR16000] - BSA and Azide free (ab251010)

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